{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:08:53Z","timestamp":1770329333054,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T00:00:00Z","timestamp":1626220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To identify clonal neoplastic cells in skin affected by B\u2010cell lymphoma using skin flow cytometry (FCM) techniques, we investigated light\u2010chain restriction using skin FCM with clonality assessed by polymerase chain reaction and light\u2010chain restriction by in situ hybridization (ISH). We retrospectively analyzed 16 cases of B\u2010cell lymphoma with cutaneous involvement: primary cutaneous diffuse large B\u2010cell lymphoma, leg type (pcDLBCL\u2010LT) (n\u00a0=\u00a07), DLBCL\u2013not otherwise specified (DLBCL\u2010NOS) (n\u00a0=\u00a06), primary cutaneous follicle center lymphoma (pcFCL) (n\u00a0=\u00a01), and follicular lymphoma (n\u00a0=\u00a02), as well as cutaneous B\u2010cell pseudolymphoma (n\u00a0=\u00a09). Results of skin FCM light\u2010chain restriction analyses were compared with immunoglobulin H (<jats:italic>IgH<\/jats:italic>) gene rearrangement and \u03ba\/\u03bb ISH findings. Skin FCM detected light\u2010chain restriction in 11 of 14 B\u2010cell lymphoma patients but none of the B\u2010cell pseudolymphoma patients. The sensitivity of skin FCM for distinguishing B\u2010cell lymphoma and B\u2010cell pseudolymphoma was 79%, and the specificity was 100%. Eleven of 13 B\u2010cell lymphoma patients exhibited gene rearrangement (sensitivity 85%), whereas six of seven pseudolymphoma patients were negative (specificity 86%). ISH was positive in three of 16 B\u2010cell lymphoma cases (sensitivity 19%) but none of the B\u2010cell pseudolymphoma cases (specificity 100%). ISH sensitivity was 29% for pcDLBCL\u2010LT, 17% for DLBCL\u2010NOS, and 0% for pcFCL and follicular lymphoma. Skin FCM therefore appears to be more sensitive than ISH in detecting light\u2010chain restriction in DLBCL and follicular lymphoma, and as sensitive as <jats:italic>IgH<\/jats:italic> gene rearrangement analysis in detecting clonality. Skin FCM is thus a promising diagnostic tool for identifying monoclonal neoplastic B\u2010cell populations.<\/jats:p>","DOI":"10.1111\/1346-8138.16057","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T05:55:46Z","timestamp":1626328546000},"page":"246-252","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of clonality in cutaneous B\u2010cell lymphoma and B\u2010cell pseudolymphoma using skin flow cytometry: Comparison of immunophenotyping and gene rearrangement studies"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6457-0307","authenticated-orcid":false,"given":"Yuki","family":"Nakagawa","sequence":"first","affiliation":[{"name":"Department of Dermatology Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4192-5707","authenticated-orcid":false,"given":"Toshihisa","family":"Hamada","sequence":"additional","affiliation":[{"name":"Department of Dermatology Takamatsu Red Cross Hospital  Takamatsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahide","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Division of Medical Support Okayama University Hospital  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Miyake","sequence":"additional","affiliation":[{"name":"Department of Dermatology Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences  Okayama 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