{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T06:25:02Z","timestamp":1776234302400,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T00:00:00Z","timestamp":1640736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K08263"],"award-info":[{"award-number":["18K08263"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A line blotting assay (LB) is currently used to detect myositis\u2010specific autoantibodies (MSAs) in patients with idiopathic inflammatory myopathies (IIMs), because of its simplicity; however, the sensitivity and specificity of this assay is low. The aim of this study is to evaluate the accuracy of the commercial LB in detection of antinuclear matrix protein 2 (NXP2) antibody. Seventy\u2010seven serum samples from patients with IIMs, in which anti\u2010NXP2 antibodies were detected through immunoprecipitation and western blotting (IP\u2010WB) using K562 cell lysate, were enrolled. All samples were assessed by LB and IP\u2010WB using recombinant human NXP2 whole protein (rNXP2) produced by insect cells, and the positive rates of each assay were compared. Thirty\u2010two samples (41.6%) showed false\u2010negativity by LB, which includes 11\u00a0samples with negative results by IP\u2010WB using rNXP2. Relative intensities of IP\u2010WB using cell lysate were significantly higher in the samples with positive results by both LB and IP\u2010WB using rNXP2, compared to samples with positive by IP\u2010WB using rNXP2 but negative by LB. Three of 11\u00a0samples with negative results by both LB and IP\u2010WB using rNXP2 revealed high antibody titers. Further, differences in post\u2010transcriptional SUMOylation were observed between recombinant and natural NXP2 proteins. In conclusion, the LB showed low sensitivity for detection of anti\u2010NXP2 antibody, an effect exacerbated at low titers of anti\u2010NXP2 antibodies. Moreover, there appears to be differences in the reactivities of antibodies to recombinant and natural NXP2 proteins with different post\u2010transcriptional modifications.<\/jats:p>","DOI":"10.1111\/1346-8138.16295","type":"journal-article","created":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T03:14:56Z","timestamp":1640834096000},"page":"441-447","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Reliability of antinuclear matrix protein 2 antibody assays in idiopathic inflammatory myopathies is dependent on target protein properties"],"prefix":"10.1111","volume":"49","author":[{"given":"Yuki","family":"Ichimura","sequence":"first","affiliation":[{"name":"Department of Dermatology Faculty of Medicine University of Tsukuba  Ibaraki Japan"},{"name":"Department of Dermatology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University  Tokyo 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