{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T04:25:38Z","timestamp":1771302338672,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T00:00:00Z","timestamp":1681948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Anti\u2010p200 pemphigoid is a rare subepidermal blistering disease showing immunoglobulin G (IgG) autoantibodies reactive with a 200\u2010kDa protein. In most patients, serum IgG antibodies react with laminin \u03b31. The diagnosis of anti\u2010p200 pemphigoid is occasionally difficult, mainly due to the lack of standardized tests. We performed fluorescence overlay antigen mapping by laser scanning confocal microscopy (FOAM\u2010LSCM) to identify autoantigens in an anti\u2010p200 pemphigoid patient and assessed its usefulness for the diagnosis. A 71\u2010year\u2010old man presented with blisters and erosions on the bilateral forearms. No mucosal lesions were observed. Laboratory examinations revealed mild leukocytosis and antinuclear antibody negativity. A histopathological examination showed subepidermal blisters with neutrophil infiltration. Direct immunofluorescence showed linear IgG staining along the basement membrane zone. Indirect immunofluorescence using 1\u2009M NaCl\u2010split skin sections revealed IgG reactivity on the dermal side. Immunoblotting detected circulating IgG autoantibodies that reacted with a 200\u2010kDa protein. Accordingly, anti\u2010p200 pemphigoid was diagnosed. FOAM\u2010LSCM revealed that the patient's IgG signals were co\u2010localized with laminin \u03b31 but were observed above type VII collagens. A direct immunofluorescent analysis for IgG deposition patterns showed an n\u2010serrated pattern. Thus, FOAM\u2010LSCM may be useful for diagnosing anti\u2010p200 pemphigoid.<\/jats:p>","DOI":"10.1111\/1346-8138.16810","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T06:34:11Z","timestamp":1682058851000},"page":"1194-1198","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Usefulness of immunofluorescence overlay antigen mapping in the identification of autoantigen in anti\u2010p200 pemphigoid"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7786-8997","authenticated-orcid":false,"given":"Shohei","family":"Kitayama","sequence":"first","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine, Academic Assembly University of Toyama  Toyama 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