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Here, we detected the skin barrier function in PCA patients and healthy people by using noninvasive techniques and characterized ultrastructural features of PCA lesions compared with healthy people using transmission electron microscopy (TEM). The expression of proteins related to skin barrier function was examined by immunohistochemistry staining. A total of 191 patients with clinically diagnosed PCA and 168 healthy individuals were enrolled in the study. Our analysis revealed that all investigated lesion areas displayed higher transepidermal water loss and pH values, and lower Sebum levels and stratum corneum hydration levels in PCA patients compared with the same site area in healthy individuals. The TEM results showed that the intercellular spaces between the basal cells were enlarged and the number of hemidesmosomes decreased in PCA lesions. Immunohistochemical staining showed that the expression of integrin \u03b16 and E\u2010cadherin in PCA patients was less than that in healthy controls, while no differences in the expression of loricrin and filaggrin were observed. Our study revealed that individuals with PCA displayed skin barrier dysfunction, which may be related to alterations in epidermal ultrastructure and a decrease in the skin barrier\u2010related protein E\u2010cadherin. However, the molecular mechanisms underlying skin barrier dysfunction in PCA remain to be elucidated.<\/jats:p>","DOI":"10.1111\/1346-8138.16819","type":"journal-article","created":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T06:47:30Z","timestamp":1683614850000},"page":"999-1007","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Barrier function and ultrastructure characteristics of epidermis in patients with primary cutaneous amyloidosis"],"prefix":"10.1111","volume":"50","author":[{"given":"Yuling","family":"Zhang","sequence":"first","affiliation":[{"name":"Dermatology Hospital Southern Medical University  Guangzhou China"},{"name":"Department of Dermatology The First Hospital of Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Le","sequence":"additional","affiliation":[{"name":"Dermatology Hospital Southern Medical University  Guangzhou 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