{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:35:02Z","timestamp":1789576502997,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["22K16271"],"award-info":[{"award-number":["22K16271"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Exophiala<\/jats:italic> species cause chromoblastomycosis, mycetoma, and phaeohyphomycosis, which are occasionally fatally in immunocompromised patients. Matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS) provides rapid and accurate examination of isolated bacteria and some fungal isolates, but the preparation method for filamentous fungi is complicated. In this study, 31 clinical isolates of <jats:italic>Exophiala<\/jats:italic> spp. in Japan were identified by MALDI\u2010TOF MS with a library enriched by adding data. To simplify the sample preparation method, two modified methods were compared with the standard method for filamentous fungi. The agar cultivation sample preparation method reduced the time required for liquid culture and was considered suitable for clinical use. In 30 of 31 clinical isolates of <jats:italic>Exophiala<\/jats:italic> spp., the species identified by MALDI\u2010TOF MS with the highest score matched the species identified by sequencing the internal transcribed spacer region. <jats:italic>Exophiala dermatitidis<\/jats:italic>, <jats:italic>E.\u2009lecanii\u2010corni<\/jats:italic>, and <jats:italic>E.\u2009oligosperma<\/jats:italic> were identified above the genus level, while <jats:italic>E.\u2009jeanselmei<\/jats:italic> and <jats:italic>E.\u2009xenobiotica<\/jats:italic> were often not identified at the species level. The identification scores tended to be lower for less\u2010registered strains in the in\u2010house library. It is suggested that library enrichment and the modified preparation method may facilitate early diagnosis of rare fungal infections by <jats:italic>Exophiala<\/jats:italic> spp. in clinical laboratories using MALDI\u2010TOF MS.<\/jats:p>","DOI":"10.1111\/1346-8138.16878","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T07:05:01Z","timestamp":1688022301000},"page":"1313-1320","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>MALDI\u2010TOF MS<\/scp> identification of <i>Exophiala<\/i> species isolated in Japan: Library enrichment and faster sample preparation"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6790-618X","authenticated-orcid":false,"given":"Taketoshi","family":"Futatsuya","sequence":"first","affiliation":[{"name":"Department of Dermatology Kanazawa Medical University  Ishikawa 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