{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T09:12:05Z","timestamp":1774516325204,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Laminin332 is a glycoprotein consisting of \u03b13\/\u03b23\/\u03b32 chains, of which the \u03b32 chain (Ln\u2010\u03b32) is expressed in tumor cells at the invasive front in many types of malignant tumors. We have previously reported that Ln\u2010\u03b32 is associated with tumor invasion of cutaneous squamous cell carcinoma (cSCC) in vivo and in vitro. Recently, tumor budding (TB; invasion patterns in small clusters of less than five cancer cells in the stroma at the invasive front) has been reported to be a risk factor for lymph node metastasis in cSCC. Based on these findings, we speculated that expression of Ln\u2010\u03b32 is related to TB in cSCC and would be an invasive factor that causes lymph node metastasis. In this study, we investigated the relationship between Ln\u2010\u03b32 expression and clinicopathological findings, including TB, in 102 cases of cSCC using immunohistochemistry. The results showed that high expression of Ln\u2010\u03b32 at the invasive front correlated with a high TB score. In addition, high Ln\u2010\u03b32 expression at the invasive front was also associated with lymphatic invasion, lymph node metastasis, and poor prognosis (death or recurrence), as in TB. Furthermore, we showed a positive association between Ln\u2010\u03b32 expression at the invasive front and Yes\u2010associated protein (YAP) expression in the Hippo pathway. Our results suggest that Ln\u2010\u03b32 expression at the invasive front may have a role in TB formation via YAP and contribute to prognosis by causing lymphatic invasion and lymph node metastasis. The expression of Ln\u2010\u03b32 would be useful for risk assessment of lymph node metastasis and poor prognosis in routine practice of cSCC.<\/jats:p>","DOI":"10.1111\/1346-8138.16952","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T06:36:04Z","timestamp":1695796564000},"page":"1585-1593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Expression of laminin332 \u03b32 at the invasive front is associated with tumor budding and poor prognosis in cutaneous squamous cell carcinoma"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9579-8266","authenticated-orcid":false,"given":"Shiori","family":"Katayama","sequence":"first","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine  Fukuoka Japan"},{"name":"Department of Dermatology Fukuoka University School of Medicine  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7103-242X","authenticated-orcid":false,"given":"Kaori","family":"Koga","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Kyoto University Hospital  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibu","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Department of Human Pathology Wakayama Medical University  Wakayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuki","family":"Nabeshima","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Pathological Diagnosis Center Fukuoka Tokushukai Hospital  Kasuga Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8568-4349","authenticated-orcid":false,"given":"Shinichi","family":"Imafuku","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fukuoka University School of Medicine  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Hamasaki","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.08.059"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2017.1680"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.09.026"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2006.00150.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.283"},{"key":"e_1_2_7_7_1","first-page":"5596","article-title":"Overexpression of laminin \u03b32 chain monomer in invading gastric carcinoma cells","volume":"59","author":"Koshikawa N","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_7_8_1","first-page":"4132","article-title":"Laminin\u20105 is a marker of invading cancer cells in some human carcinomas and is coexpressed with the receptor for urokinase plasminogen activator in budding cancer cells in colon adenocarcinomas","volume":"55","author":"Pyke C","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_7_9_1","first-page":"896","article-title":"Expression of the \u03b32 chain of laminin\u20105 at the invasive front is associated with recurrence and poor prognosis in human esophageal squamous cell carcinoma","volume":"7","author":"Yamamoto H","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-015-1620-4"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2017.46"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001284"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12530"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-019-1082-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.05.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-020-03388-3"},{"key":"e_1_2_7_17_1","first-page":"24","volume-title":"WHO classification of skin tumours","author":"Scolyer RA","year":"2018"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2017.04.004"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2006.00621.x"},{"key":"e_1_2_7_20_1","volume-title":"Japanese classification of colorectal, appendiceal, and anal carcinoma","author":"Japanese Society for Cancer of the Colon and Rectum","year":"2018"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aay3324"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20021454"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.03871.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2017.01.010"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32027"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12323"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.12752"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm5050051"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20001201)88:5<708::AID-IJC5>3.0.CO;2-J"},{"key":"e_1_2_7_30_1","first-page":"8089","article-title":"Expression of the invasion factor laminin \u03b32 in colorectal carcinomas is regulated by \u03b2\u2010catenin","volume":"61","author":"Hlubek F","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.02.005"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S85050"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.23964"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14725"}],"container-title":["The Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.16952","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T14:21:02Z","timestamp":1730902862000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1346-8138.16952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,26]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/1346-8138.16952"],"URL":"https:\/\/doi.org\/10.1111\/1346-8138.16952","archive":["Portico"],"relation":{},"ISSN":["0385-2407","1346-8138"],"issn-type":[{"value":"0385-2407","type":"print"},{"value":"1346-8138","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,26]]},"assertion":[{"value":"2023-07-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}