{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T05:26:17Z","timestamp":1772601977764,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acne vulgaris is a complex condition involving factors that affect the pilosebaceous unit. A primary manifestation of acne pathology is the development of comedones, often linked to the overproduction of sebum resulting from 5\u03b1\u2010dihydrotestosterone (5\u03b1\u2010DHT) and insulin activity. Ozenoxacin is a topical quinolone that exhibits potent antibacterial activity against <jats:italic>Cutibacterium acnes<\/jats:italic> (<jats:italic>C.\u2009acnes<\/jats:italic>). It is commonly used to treat acne associated with this bacterium; however, its effect on sebum production within the sebaceous glands remains unclear. In this study, the effects of ozenoxacin on sebum production were examined using insulin\u2010 and 5\u03b1\u2010DHT\u2010differentiated hamster sebocytes. Ozenoxacin showed a dose\u2010dependent inhibition of lipid droplet formation and triacylglycerol (TG) production, which is a major component of sebum. In addition, it suppressed the expression of diacylglycerol acyltransferase 1, stearoyl\u2010CoA desaturase\u20101, and perilipin\u20101 mRNA, all important factors involved in sebum synthesis, in a dose\u2010dependent manner. Moreover, ozenoxacin decreased phosphorylated 40S ribosomal protein S6 levels downstream of the mechanistic\/mammalian target of rapamycin complex 1 (mTORC1), without altering the phosphorylation of Akt, an upstream regulator of mTORC1, in both insulin\u2010 and 5\u03b1\u2010DHT\u2010treated hamster sebocytes. Interestingly, nadifloxacin, but not clindamycin, exhibited a similar suppression of sebum production, albeit with lesser potency compared with ozenoxacin. Furthermore, a topical application of a 2% ozenoxacin\u2010containing lotion to the auricle skin of hamsters did not affect the size of the sebaceous glands or epidermal thickness. Notably, it decreased the amount of TG on the skin surface. The results provide novel insights into the sebum\u2010inhibitory properties of ozenoxacin, indicating its potential efficacy in controlling microbial growth and regulating sebum production for acne management.<\/jats:p>","DOI":"10.1111\/1346-8138.17409","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T12:52:33Z","timestamp":1722516753000},"page":"1187-1198","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ozenoxacin suppresses sebum production by inhibiting <scp>mTORC1<\/scp> activation in differentiated hamster sebocytes"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1966-8527","authenticated-orcid":false,"given":"Takamichi","family":"Kitano","sequence":"first","affiliation":[{"name":"Drug Development Research Laboratories, Kyoto R&amp;D Center Maruho Co., Ltd.  Kyoto Japan"},{"name":"Department of Biochemistry, School of Pharmacy, School of Pharmacy Tokyo University of Pharmacy and Life Sciences  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshikazu","family":"Koiwai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Pharmacy, School of Pharmacy Tokyo University of Pharmacy and Life Sciences  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koki","family":"Fujikawa","sequence":"additional","affiliation":[{"name":"Strategic Research Planning &amp; Management Department, Maruho Shonan Innovation Lab Maruho Co., Ltd.  Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachi","family":"Mori","sequence":"additional","affiliation":[{"name":"Drug Development Research Laboratories, Kyoto R&amp;D Center Maruho Co., Ltd.  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