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Advances in long\u2010read sequencing technologies have significantly enhanced our ability to accurately determine haplotypes. This review discusses the application of low\u2010coverage long\u2010read sequencing, nanopore Cas9\u2010guided long\u2010read sequencing, and adaptive sampling in phasing, highlighting their utility in complex clinical scenarios. Through clinical vignettes, we explore the importance of phasing in gene therapy design for recessive dystrophic epidermolysis bullosa and the role of revertant mosaicism in therapeutic epidermal autografts. Despite its promise, phasing with long\u2010read sequencing faces challenges, including low efficiency in enriching target regions and the inherent error rate of nanopore sequencing. Future developments in long\u2010read sequencing technologies will be critical in overcoming these limitations and expanding the applicability of phasing across various clinical settings.<\/jats:p>","DOI":"10.1111\/1346-8138.17597","type":"journal-article","created":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T10:19:33Z","timestamp":1735208373000},"page":"392-399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advanced phasing techniques in congenital skin diseases"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3865-6366","authenticated-orcid":false,"given":"Ken","family":"Natsuga","sequence":"first","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine and Graduate School of Medicine Hokkaido University  Sapporo 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