{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T09:15:59Z","timestamp":1788513359970,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recently, cell\u2010free DNA (cfDNA) has gained attention as a diagnostic and prognostic biomarker for various conditions, including autoimmune and inflammatory skin diseases. However, reports of cfDNA use in patients with systemic sclerosis (SSc)\u00a0are limited. The purpose of this study was to investigate the clinical significance of cfDNA in patients with SSc and assess the circulating DNA levels of key cytokines associated with SSc fibrosis. Serum samples were obtained from 64 patients with SSc, 9 with very early SSc, and 20 healthy controls (HC). Circulating DNA copies were quantified using droplet digital polymerase chain reaction analysis. The DNA copies of interleukin\n                    <jats:italic>(IL)\u20106<\/jats:italic>\n                    ,\n                    <jats:italic>IL\u20108<\/jats:italic>\n                    , Janus kinase 2 (\n                    <jats:italic>JAK2<\/jats:italic>\n                    ), and connective tissue growth factor were significantly higher in patients with SSc than in the HCs. Although not statistically significant, circulating\n                    <jats:italic>IL<\/jats:italic>\n                    \u20106 DNA copies were higher in patients with SSc than in patients with very early SSc. Receiver operating characteristic analysis indicated that\n                    <jats:italic>IL\u20106<\/jats:italic>\n                    DNA copies could distinguish patients with SSc from both the HCs and patients with very early SSc (area under the curve &gt;\u20090.7 in both cases). Disease type dcSSc and reduced %FVC were significant independent predictors of higher circulating\n                    <jats:italic>IL\u20106<\/jats:italic>\n                    and\n                    <jats:italic>JAK2<\/jats:italic>\n                    DNA copies in multivariate logistic regression analysis. cfDNA, including circulating\n                    <jats:italic>IL\u20106<\/jats:italic>\n                    and\n                    <jats:italic>JAK2<\/jats:italic>\n                    DNA in sera, may serve as valuable indicators for diagnosis and fibrosis progression in patients with SSc.\n                  <\/jats:p>","DOI":"10.1111\/1346-8138.17857","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T12:28:39Z","timestamp":1752236919000},"page":"1430-1438","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Circulating\n                    <i>Interleukin\u20106<\/i>\n                    and\n                    <i>Janus Kinase 2<\/i>\n                    <scp>DNA<\/scp>\n                    Copies in Serum Cell\u2010Free\n                    <scp>DNA<\/scp>\n                    Are Elevated in Patients With Systemic Sclerosis"],"prefix":"10.1111","volume":"52","author":[{"given":"Hitomi","family":"Matsunaga\u2010Nakashima","sequence":"first","affiliation":[{"name":"Department of Dermatology and Plastic Surgery, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1697-9316","authenticated-orcid":false,"given":"Soichiro","family":"Sawamura","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8381-2386","authenticated-orcid":false,"given":"Tselmeg Mijiddorj","family":"Myangat","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0096-1613","authenticated-orcid":false,"given":"Tamami","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8900-3520","authenticated-orcid":false,"given":"Shuichi","family":"Shimada","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery, Faculty of Life Sciences Kumamoto University  Kumamoto 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