{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T08:41:16Z","timestamp":1778575276591,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to investigate the prevalence of co\u2010inhibitory molecules (CIMs) on lymphocytes in patients with psoriasis (PsO). Combining bioinformatics analysis and experimental validation, we first analyzed the GSE109248 dataset to characterize lymphocyte immunoinfiltration patterns and CIM\u2010related gene expression profiles in PsO patients compared with healthy donors (HDs). Subsequently, peripheral blood mononuclear cells (PBMCs) from 15 PsO patients and 8 HDs were systematically examined to quantify surface expression levels of TIGIT\/CD226, PD\u20101, LAG\u20103, and TIM\u20103 across various T cell subpopulations and NK cells, thereby complementing the transcriptomic findings with functional cellular insights. The Results show that no notable differences were observed in the levels of NK cells, CD4\n                    <jats:sup>+<\/jats:sup>\n                    , and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells when comparing PsOs to HDs in the GEO dataset and clinical samples. However, blood samples indicated a significant rise in CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    effector memory T cells (TEM) in PsOs compared to HDs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Further examination showed an increase in the proportion of TIGIT\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    TEMs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), while that of TIM\u20103\n                    <jats:sup>+<\/jats:sup>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    TEMs exhibited a decrease (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The percentages of CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    TEM cells expressing CD226 were significantly elevated in PsOs compared to HDs (both\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), whereas those expressing CD62L were significantly diminished (both\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Additionally, the ratio of PD\u20101\n                    <jats:sup>+<\/jats:sup>\n                    NK was increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Subgroup analysis between various degrees of disease severity in PsO patients showed that compared with those with mild psoriasis, patients with moderate to severe psoriasis had a decrease in the ratio of TEM in CD4+ and CD8+ T cells (both\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), an increase in percentage and MIF of TEM with CD62L expression in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells (both\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), a higher MIF of TIM\u20103 expression on CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) and a higher MIF of PD\u20101 on NK cells (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The differential expression of TIGIT\/CD226 on CD8\n                    <jats:sup>+<\/jats:sup>\n                    TEM could indicate ongoing activation of lymphocytes. The extremely decreased expression of CD62L\n                    <jats:sup>+<\/jats:sup>\n                    TEM cells may indicate a significant shedding of CD62L from immune cells, which may suggest a release of lymphocytes into serum and aggravate systemic inflammation of PsO. However, the PD\u20101 expression model on NK cells may necessitate additional research within a larger population for its significance in psoriasis. The relatively small sample size (15 PsO patients and 8 healthy donors) may limit the statistical power and generalizability of the findings; thus, larger cohorts are warranted to confirm these results.\n                  <\/jats:p>","DOI":"10.1111\/1346-8138.17937","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T07:40:36Z","timestamp":1758699636000},"page":"1698-1708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression of Co\u2010Inhibitory Molecules on Lymphocytes of Patients With Psoriasis"],"prefix":"10.1111","volume":"52","author":[{"given":"Guanfang","family":"Liu","sequence":"first","affiliation":[{"name":"Guangzhou Dermatology Hospital  Guangzhou Guangdong China"},{"name":"Sino\u2010French Hoffmann Institute, School of Basic Medical Sciences, The Second Affiliated Hospital of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy &amp; Clinical Immunology Guangzhou Medical University  Guangzhou Guangdong China"},{"name":"Department of Emergency Medicine The Affiliated Guangdong Second Provincial General Hospital of Jinan University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Huang","sequence":"additional","affiliation":[{"name":"Sino\u2010French Hoffmann Institute, School of Basic Medical Sciences, The Second Affiliated Hospital of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy &amp; Clinical Immunology Guangzhou Medical University  Guangzhou Guangdong China"},{"name":"Department of Clinical Laboratory Maoming Hospital of Guangzhou University of Chinese Medicine  Maoming Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Jing","family":"Gao","sequence":"additional","affiliation":[{"name":"Hospital for Skin Diseases, Institute of Dermatology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui\u2010Lian","family":"Ding","sequence":"additional","affiliation":[{"name":"Hospital for Skin Diseases, Institute of Dermatology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Nanjing Jiangsu China"},{"name":"Qingdao Municipal Center for Disease Control &amp; Prevention  Qingdao Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2597-2132","authenticated-orcid":false,"given":"Yu","family":"Zheng","sequence":"additional","affiliation":[{"name":"Hospital for Skin Diseases, Institute of Dermatology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Ping","family":"Tang","sequence":"additional","affiliation":[{"name":"Guangzhou Dermatology Hospital  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8555-0922","authenticated-orcid":false,"given":"Jin","family":"Bu","sequence":"additional","affiliation":[{"name":"Hospital for Skin Diseases, Institute of Dermatology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Er\u2010Xia","family":"Shen","sequence":"additional","affiliation":[{"name":"Sino\u2010French Hoffmann Institute, School of Basic Medical Sciences, The Second Affiliated Hospital of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy &amp; Clinical Immunology Guangzhou Medical University  Guangzhou Guangdong China"},{"name":"Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, School of Basic Medical Sciences Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200201151-00011"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.717042"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12518"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgaa701"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.currproblcancer.2017.10.003"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.11.021"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.04.012"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100643"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.107722"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22115497"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/JD9.0000000000000074"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2002.01721.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09178.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.5582\/bst.2022.01501"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201948405"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.840457"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2022-004711"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002835"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2016.10.002"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.02.005"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.09.010"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-020-02397-4"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(89)90151-7"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/101879"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/immadv\/ltaa004"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/ced.13414"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2014.10.018"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-01308-2"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2100240"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13027"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.5826\/dpc.1302a134"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2019.101302"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903435"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1285"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12715"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-024-01024-7"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01068"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.8.4120"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01404.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1403680"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-019-0206-4"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI99317"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvh.13352"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxab085"}],"container-title":["The Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.17937","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1346-8138.17937","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.17937","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:52:21Z","timestamp":1773003141000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1346-8138.17937"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,24]]},"references-count":44,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/1346-8138.17937"],"URL":"https:\/\/doi.org\/10.1111\/1346-8138.17937","archive":["Portico"],"relation":{},"ISSN":["0385-2407","1346-8138"],"issn-type":[{"value":"0385-2407","type":"print"},{"value":"1346-8138","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,24]]},"assertion":[{"value":"2025-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}