{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:38:50Z","timestamp":1773009530887,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Argyria, characterized by irreversible bluish\u2010gray skin pigmentation due to silver accumulation, presents significant cosmetic concerns. Scanning transmission electron microscopy (STEM) combined with energy\u2010dispersive X\u2010ray spectroscopy (EDS) allows for precise identification of silver particles in tissue; however, standard specimen preparation protocols are labor\u2010intensive. This study evaluates the feasibility, diagnostic utility, and limitations of a simplified STEM\u2010EDS method using conventionally prepared transmission electron microscopy (TEM) specimens. A 72\u2010year\u2010old man with a two\u2010year history of ingesting silver\u2010ionized water three times daily presented with diffuse blue\u2010brown pigmentation of the facial skin and nails. Based on clinical suspicion of systemic argyria, skin biopsy samples were analyzed using STEM\u2010EDS on ultrathin sections prepared via standard TEM protocols. Granules approximately 200\u2013400\u2009nm in diameter exhibited distinct EDS emission peaks for silver and selenium. Elemental line scans revealed silver as the primary component, and areal mapping confirmed the co\u2010localization of silver and selenium within the same granules. This case highlights the diagnostic utility of STEM\u2010EDS in confirming silver deposition in suspected systemic argyria, even when using standard TEM\u2010prepared samples. The streamlined approach eliminates the need for additional specimen processing while maintaining analytical integrity.<\/jats:p>","DOI":"10.1111\/1346-8138.17942","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T08:51:28Z","timestamp":1757667088000},"page":"1741-1745","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Argyria Induced by Prolonged Ingestion of Silver Ionized Water: A Scanning Transmission Electron Microscopy With Energy\u2010Dispersive X\u2010Ray Analysis Utilizing a Simplified Sample Preparation Method"],"prefix":"10.1111","volume":"52","author":[{"given":"Yuta","family":"Ito","sequence":"first","affiliation":[{"name":"Department of Dermatology Showa Medical University Fujigaoka Hospital  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiro","family":"Okusa","sequence":"additional","affiliation":[{"name":"Department of Dermatology Showa Medical University Fujigaoka Hospital  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4963-9337","authenticated-orcid":false,"given":"Tokio","family":"Nakada","sequence":"additional","affiliation":[{"name":"Department of Dermatology Showa Medical University Fujigaoka Hospital  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5691-7211","authenticated-orcid":false,"given":"Hirohiko","family":"Sueki","sequence":"additional","affiliation":[{"name":"Department of Dermatology, School of Medicine Showa Medical University  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000093928"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2004.09.026"},{"issue":"2","key":"e_1_2_8_4_1","first-page":"245","article-title":"Systemic Argyria Secondary to Breath Freshener \u201cJintan Silver Pills\u201d","volume":"36","author":"Shimamoto Y.","year":"1987","journal-title":"Hiroshima Journal of Medical Sciences"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(05)60616\u20102"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102133.1999.02628.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365518109092041"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346\u20108138.1992.tb03186.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104613\u20102529\u20101_9"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100773.1986.tb02805.x"},{"key":"e_1_2_8_11_1","unstructured":"Japan ION Corporation \u201cAbout Silver\u2010Ionic Water \u201d(2006) https:\/\/www.japan\u2010ion.com\/silver\/agwater.html."},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440\u20101819.1996.tb01669.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.6.556"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.114.3.373"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.5070\/D30832G6D3"},{"issue":"5","key":"e_1_2_8_16_1","first-page":"373","article-title":"Argyria Following the Use of Dietary Supplements Containing Colloidal Silver Protein","volume":"66","author":"Gulbranson S. H.","year":"2000","journal-title":"Cutis"}],"container-title":["The Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.17942","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1346-8138.17942","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.17942","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:52:27Z","timestamp":1773003147000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1346-8138.17942"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,12]]},"references-count":15,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/1346-8138.17942"],"URL":"https:\/\/doi.org\/10.1111\/1346-8138.17942","archive":["Portico"],"relation":{},"ISSN":["0385-2407","1346-8138"],"issn-type":[{"value":"0385-2407","type":"print"},{"value":"1346-8138","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,12]]},"assertion":[{"value":"2025-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}