{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T12:42:11Z","timestamp":1785156131274,"version":"3.55.0"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Transformed mycosis fungoides (TMF) is a rare, aggressive variant of cutaneous T\u2010cell lymphoma characterized by the presence of large neoplastic cells and poor clinical outcomes. A retrospective cohort of 22 TMF patients was analyzed using immunohistochemistry on formalin\u2010fixed, paraffin\u2010embedded (FFPE) tissue for GATA3 (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920), T\u2010bet (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200922), and STAT3 (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200922). Expression was quantified by image analysis integrated optical density per total area (IOD\/area), standardized by\n                    <jats:italic>z<\/jats:italic>\n                    \u2010score and correlated with survival. Seventeen patients with complete data underwent unsupervised clustering (\n                    <jats:italic>k<\/jats:italic>\n                    \u2010means) and principal component analysis (PCA) based on marker expression profiles. High GATA3 expression was strongly associated with worse prognosis (median OS: 8.6\u2009months vs. 41.7\u2009months,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.00094). T\u2010bet and STAT3 expressions showed no significant individual association with survival. Clustering analysis revealed three distinct immunoprofiles: (1) low expression of all markers (intermediate survival, 28.1\u2009months), (2) high STAT3 and T\u2010bet expressions with intermediate GATA3 expression (longest survival, 53.1\u2009months), and (3) high GATA3 expression with low STAT3 and T\u2010bet expressions (poorest survival, 9.5\u2009months). GATA3 is a robust prognostic marker in TMF, identifying patients with particularly poor outcomes. Its elevated expression delineates a Th2\u2010skewed, immunosuppressive phenotype that may inhibit Th1\/Th17 pathways via transcriptional repression. Integrative profiling reveals immunobiological subgroups with divergent prognoses, supporting GATA3 as a potential tool for risk stratification and a candidate for targeted intervention in TMF.\n                  <\/jats:p>","DOI":"10.1111\/1346-8138.70070","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T07:51:13Z","timestamp":1766130673000},"page":"304-308","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunoprofiling Reveals\n                    <scp>GATA3<\/scp>\n                    as a Prognostic Marker in Transformed Mycosis Fungoides"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7140-3462","authenticated-orcid":false,"given":"Bruno de Castro e","family":"Souza","sequence":"first","affiliation":[{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1959-4908","authenticated-orcid":false,"given":"Denis","family":"Miyashiro","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9741-4311","authenticated-orcid":false,"given":"Jade","family":"Cury\u2010Martins","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neusa Yuriko Sakai","family":"Valente","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Fernando Ferraz","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Pathology University of S\u00e3o Paulo Medical School  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5709-092X","authenticated-orcid":false,"given":"Jos\u00e9 Antonio","family":"Sanches","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102024\u2010209213"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2021.07.013"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13225854"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408\u2010020\u201000380\u20105"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13050419"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201012\u20103488"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2014.09.022"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.4.1150"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0000000000000166"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.71151"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408\u2010022\u201000745\u2010y"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.6793"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346\u20108138.17917"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20191761"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413461200"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500335"}],"container-title":["The 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