{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T07:55:27Z","timestamp":1791446127052,"version":"4.3.3"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigated the association between CD73 expression and malignant progression in NF1\u2010associated peripheral nerve sheath tumors and evaluated its potential as a diagnostic marker. Histopathological differentiation of atypical neurofibromatous neoplasms of uncertain biological potential (ANNUBP) and low\u2010grade malignant peripheral nerve sheath tumors (MPNST) in patients with NF1 remains challenging. Therefore, reliable adjunctive markers are needed to improve diagnostic accuracy. CD73 is an ectoenzyme with ecto\u20105\u2032\u2010nucleotidase activity. Recent studies have also suggested that CD73 may promote tumor invasion by facilitating extracellular matrix remodeling, independent of immune suppression. To investigate the role of CD73 in NF1\u2010associated tumors, we analyzed CD73 expression in 57 NF1\u2010associated tumors, including 39 benign neurofibromas (NF), 7 ANNUBP, and 11 MPNST, using immunohistochemistry and reverse transcription\u2010 quantitative PCR (RT\u2010qPCR) in selected cases. Most tumors arose in the skin or subcutaneous tissue. Among the four histological subtypes of NF, solitary and diffuse types were mostly negative for CD73 expression (19\/20 cases), whereas plexiform and mixed types, which are associated with a higher risk of malignant transformation, more frequently exhibited positive expression. Most ANNUBP cases (6\/7 cases) demonstrated low or negative CD73 expression, whereas all MPNST cases were positive, with the majority showing high expression (9\/11 cases). CD73 expression was significantly higher in MPNST than in solitary and diffuse types of NF and ANNUBP. RT\u2010qPCR analysis consistently demonstrated a stepwise increase in\n                    <jats:italic>NT5E<\/jats:italic>\n                    (CD73) mRNA expression progressing from benign solitary and diffuse types of NF (0.74\u2009\u00b1\u20090.33) to ANNUBP (2.93\u2009\u00b1\u20090.57) and MPNST (9.26\u2009\u00b1\u20094.89). These findings suggest that CD73 expression increases in association with higher tumor grade and malignant progression in NF1\u2010associated peripheral nerve sheath tumors and may serve as a useful adjunctive marker in pathological diagnosis, particularly in cases where distinguishing ANNUBP from MPNST is challenging.\n                  <\/jats:p>","DOI":"10.1111\/1346-8138.70428","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:55:12Z","timestamp":1785131712000},"page":"1507-1514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CD73<\/scp>\n                    Expression Correlates With Malignant Progression in\n                    <scp>NF1<\/scp>\n                    \u2010Associated Peripheral Nerve Sheath Tumors: Potential Role in Pathological Diagnosis"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4857-4244","authenticated-orcid":false,"given":"Ai","family":"Matsuda","sequence":"first","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and Faculty of Medicine  Fukuoka Japan"},{"name":"Department of Dermatology Fukuoka University Faculty of Medicine  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7103-242X","authenticated-orcid":false,"given":"Kaori","family":"Koga","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and Faculty of Medicine  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9902-1383","authenticated-orcid":false,"given":"Mikiko","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and Faculty of Medicine  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5516-4737","authenticated-orcid":false,"given":"Yuichi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and Faculty of Medicine  Fukuoka Japan"},{"name":"Fukuoka Pathology Diagnostic Clinic  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3238-5297","authenticated-orcid":false,"given":"Monji","family":"Koga","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fukuoka Sanno Hospital  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Ehime University Hospital  Ehime Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2481-3888","authenticated-orcid":false,"given":"Makoto","family":"Hamasaki","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and Faculty of Medicine  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8568-4349","authenticated-orcid":false,"given":"Shinichi","family":"Imafuku","sequence":"additional","affiliation":[{"name":"Department of Dermatology Fukuoka University Faculty of Medicine  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2017.05.010"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.39.5.311"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-022-02290-1"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19860515)57:10<2006::AID-CNCR2820571022>3.0.CO;2-6"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noy013"},{"key":"e_1_2_10_7_1","first-page":"593","volume-title":"The WHO Classification of Tumors Editorial Board Skin Tumors","author":"Shea C. R.","year":"2025"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12061463"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65507-1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2017.12.015"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2015.134"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2017.97"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddz095"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-19-1429"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noae235"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15452"},{"key":"e_1_2_10_17_1","first-page":"213","article-title":"CD73 a Novel Marker for the Diagnosis of Benign and Malignant Salivary Gland Tumors","volume":"11","author":"Ranjbar M.","year":"2019","journal-title":"Journal of Clinical and Experimental Dentistry"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.5114\/pjp.2022.125537"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.2217\/imt.15.106"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-6127-x"},{"key":"e_1_2_10_21_1","first-page":"1359","article-title":"Expression of Emmprin and Matrix Metalloproteinases (MMPs) in Peripheral Nerve Sheath Tumors: Emmprin and Membrane\u2010Type (MT)1\u2010MMP Expressions Are Associated With Malignant Potential","volume":"26","author":"Nabeshima K.","year":"2006","journal-title":"Anticancer Research"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2020-001467"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/noajnl\/vdz047"},{"key":"e_1_2_10_24_1","first-page":"2123","article-title":"Immunohistochemical CD73 Expression Status in Gastrointestinal Neuroendocrine Neoplasms: A Retrospective Study of 136 Patients","volume":"15","author":"Ono K.","year":"2018","journal-title":"Oncology Letters"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-017-3111-1"}],"container-title":["The Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.70428","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1346-8138.70428","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1346-8138.70428","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T07:35:47Z","timestamp":1791444947000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1346-8138.70428"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,26]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/1346-8138.70428"],"URL":"https:\/\/doi.org\/10.1111\/1346-8138.70428","archive":["Portico"],"relation":{},"ISSN":["0385-2407","1346-8138"],"issn-type":[{"value":"0385-2407","type":"print"},{"value":"1346-8138","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,26]]},"assertion":[{"value":"2026-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}