{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:46:10Z","timestamp":1792853170564,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,15]],"date-time":"2013-04-15T00:00:00Z","timestamp":1365984000000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>The role of fibronectin binding protein A (FbpA) in <jats:italic>Listeria monocytogenes<\/jats:italic> infection and its pathogenesis were studied <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> by constructing a <jats:italic>fbpA<\/jats:italic>\u2010deficient mutant of <jats:italic>L. monocytogenes<\/jats:italic> (<jats:italic>\u0394fbpA<\/jats:italic>). <jats:italic>In vivo, \u0394fbpA<\/jats:italic> was less pathogenic in mutant mice than was wild\u2010type <jats:italic>L. monocytogenes<\/jats:italic>. FbpA did not affect the amounts of various virulence\u2010determining factors, including internalin B and listeriolysin O. However, adherence to, and invasion of, mouse hepatocytes by the <jats:italic>\u0394fbpA<\/jats:italic> mutant were reduced. In contrast, adherence to, but not invasion of, the <jats:italic>\u0394fbpA<\/jats:italic> mutant to macrophages was attenuated. Fibronectin contributed to the efficient adherence and invasion of wild\u2010type <jats:italic>L. monocytogenes<\/jats:italic>, but not to those of the <jats:italic>\u0394fbpA<\/jats:italic> mutant. Attenuation of adhesion and uptake of the <jats:italic>\u0394fbpA<\/jats:italic> mutant were reversed by overexpression of FbpA in it. FbpA was not involved in intracellular growth, autophagy induction or actin tail formation. Thus, the present findings clearly show that FbpA acts as an important adhesion molecule of <jats:italic>L. monocytogenes<\/jats:italic>, especially regarding hepatocytes, without modulating the expression of other virulence factors that have been implicated in the pathogenesis of <jats:italic>L. monocytogenes<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12030","type":"journal-article","created":{"date-parts":[[2013,4,16]],"date-time":"2013-04-16T02:54:24Z","timestamp":1366080864000},"page":"253-262","source":"Crossref","is-referenced-by-count":36,"title":["Fibronectin\u2010binding protein, FbpA, is the adhesin responsible for pathogenesis of <i>Listeria monocytogenes<\/i> infection"],"prefix":"10.1111","volume":"57","author":[{"given":"Arihiro","family":"Osanai","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Hirosaki University Graduate School of Medicine 5 Zaifu\u2010cho, Hirosaki Aomori 036\u20108562 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