{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:25:56Z","timestamp":1785774356143,"version":"3.56.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,15]],"date-time":"2013-04-15T00:00:00Z","timestamp":1365984000000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The NfxC\u2010type mutant of <jats:italic>Pseudomonas aeruginosa<\/jats:italic> produces the MexEF\u2010OprN efflux pump and down\u2010regulates expression of the quorum\u2010sensing\u2010dependent efflux pump MexAB\u2010OprM and production of virulence factors in the presence of an active transcriptional regulator, MexT. Consequently, these cells are resistant to chloramphenicol and hypersusceptible to \u03b2\u2010lactam antibiotics. An upper negative regulator, MexS, has been assumed to inactivate MexT in wild\u2010type strains, hence shutting down production of the MexEF\u2010OprN pump. This observation was, however, reported in only one clinical strain and not confirmed in well\u2010characterized laboratory strains. Moreover, it is not known whether MexS is involved in the quorum\u2010sensing\u2010dependent regulation of virulence factor production. To assess these issues, a plasmid carrying wild\u2010type <jats:italic>mexS<\/jats:italic> was introduced into three NfxC\u2010type mutants from laboratory strains, which carry an impaired <jats:italic>mexS<\/jats:italic> and unimpaired <jats:italic>mexT<\/jats:italic>. Unexpectedly, all the transformants produced an increased amount of MexEF\u2010OprN proteins. Three clinical NfxC strains were similarly transformed and although MexEF\u2010OprN was undetectable in two of these strains, one produced an increased amount of these proteins, similar to the laboratory strains. These results were interpreted to mean that <jats:italic>P. aeruginosa<\/jats:italic> takes two separate routes in MexT\u2010mediated regulation of <jats:italic>mexEF\u2010oprN<\/jats:italic> expression: the MexS\u2010bypassed pathway and MexS\u2010mediated pathway. On the other hand, the transformants of both the laboratory and clinically derived NfxC\u2010type cells produced increased amounts of MexAB\u2010OprM and virulence factors, suggesting that production of these proteins occurs via the MexS\u2010mediated pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12032","type":"journal-article","created":{"date-parts":[[2013,4,16]],"date-time":"2013-04-16T02:54:24Z","timestamp":1366080864000},"page":"263-272","source":"Crossref","is-referenced-by-count":40,"title":["Two routes of MexS\u2010MexT\u2010mediated regulation of MexEF\u2010OprN and MexAB\u2010OprM efflux pump expression in <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1111","volume":"57","author":[{"given":"Maki","family":"Uwate","sequence":"first","affiliation":[{"name":"Institute of Technology and Science The University of Tokushima Graduate School, 2\u20101 Minamijosanjima\u2010cho Tokushima 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