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This organism can survive in eukaryotic host cells by escaping reactive oxygen species via the regulation of stress responsive sigma factors, including RpoS. In <jats:italic>B. pseudomallei<\/jats:italic>, RpoS has been reported to play a role in the oxidative stress response through enhanced activity of OxyR and catalase. In this study, the RpoS dependent oxidative stress responsive system was further characterized using comparative proteomic analysis. The proteomic profiles of wild\u2010type <jats:italic>B. pseudomallei<\/jats:italic> following exposure to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and between wild\u2010type and the <jats:italic>rpo<\/jats:italic>S mutant strains were analyzed. Using stringent criteria, 13 oxidative responsive proteins, eight of which are regulated by RpoS, were identified with high confidence. It was observed that ScoA, a subunit of the SCOT enzyme not previously shown to be involved directly in the oxidative stress response, is significantly down\u2010regulated after hydrogen peroxide treatment. ScoA and ScoB have been predicted to be organized in a single operon using computational methods: in this study it was confirmed by RT\u2010PCR that these genes are indeed co\u2010transcribed as a single mRNA. The present study is the first to report a role for RpoS in the down\u2010regulation of SCOT expression in response to oxidative stress in <jats:italic>B. pseudomallei<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12077","type":"journal-article","created":{"date-parts":[[2013,6,29]],"date-time":"2013-06-29T12:18:50Z","timestamp":1372508330000},"page":"605-615","source":"Crossref","is-referenced-by-count":17,"title":["Rpo<scp>S<\/scp> and oxidative stress conditions regulate succinyl\u2010<scp>C<\/scp>o<scp>A<\/scp>: 3\u2010ketoacid\u2010coenzyme A transferase (<scp>SCOT<\/scp>) expression in <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1111","volume":"57","author":[{"given":"Palatip","family":"Chutoam","sequence":"first","affiliation":[{"name":"Department of Biochemistry Mahidol University Nakhon Pathom 73170 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