{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T20:47:21Z","timestamp":1782938841566,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,10,13]],"date-time":"2013-10-13T00:00:00Z","timestamp":1381622400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>\u03b3\u2010Glutamyltranspeptidase and asparaginase have been shown to play important roles in <jats:italic>Helicobacter pylori<\/jats:italic> colonization and cell death induced by <jats:italic>H. pylori<\/jats:italic> infection. In this study, the association of \u03b3\u2010glutamyltranspeptidase and asparaginase was elucidated by comparing activities of both deamidases in <jats:italic>H. pylori<\/jats:italic> strains from patients with chronic gastritis, gastric and duodenal ulcers, and gastric cancer. \u03b3\u2010Glutamyltranspeptidase activities in <jats:italic>H. pylori<\/jats:italic> strains from patients with gastric cancer were significantly higher than in those from patients with chronic gastritis or gastric ulcers. There was a wide range of asparaginase activities in <jats:italic>H. pylori<\/jats:italic> strains from patients with gastric cancer and these were not significantly than those from patients with other diseases. To identify the contributions of \u03b3\u2010glutamyltranspeptidase and asparaginase to gastric cell inflammation, human gastric epithelial cells (AGS line) were infected with <jats:italic>H. pylori<\/jats:italic> wild\u2010type and knockout strains and inflammatory responses evaluated by induction of interleukin\u20108 (IL\u20108). IL\u20108 response was significantly decreased by knockout of the \u03b3\u2010glutamyltranspeptidase\u2010encoding gene but not by knockout of the asparaginase\u2010encoding gene. Additionally, IL\u20108 induction by infection with the <jats:italic>H. pylori<\/jats:italic> wild\u2010type strain was significantly decreased by adding glutamine during infection. These findings indicate that IL\u20108 induction caused by \u03b3\u2010glutamyltranspeptidase activity in <jats:italic>H. pylori<\/jats:italic> is mainly attributable to depletion of glutamine. These data suggest that \u03b3\u2010glutamyltranspeptidase plays a significant role in the chronic inflammation caused by <jats:italic>H. pylori<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12089","type":"journal-article","created":{"date-parts":[[2013,8,12]],"date-time":"2013-08-12T10:02:47Z","timestamp":1376301767000},"page":"665-673","source":"Crossref","is-referenced-by-count":19,"title":["Role of \u03b3\u2010glutamyltranspeptidase in the pathogenesis of <i>Helicobacter pylori<\/i> infection"],"prefix":"10.1111","volume":"57","author":[{"given":"Emiko","family":"Rimbara","sequence":"first","affiliation":[{"name":"Department of Bacteriology II National Institute of Infectious Diseases, 4\u20107\u20101 Gakuen Musashimurayama\u2010Shi Tokyo 208\u20100011 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigetarou","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II National Institute of 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