{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:51:54Z","timestamp":1773273114895,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,11,13]],"date-time":"2013-11-13T00:00:00Z","timestamp":1384300800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>The protective efficacy of and immune response to heat\u2010killed cells of monovalent and hexavalent mixtures of six serogroups\/serotypes of <jats:italic>Shigella<\/jats:italic> strains (<jats:italic>Shigella dysenteriae<\/jats:italic> 1, <jats:italic>Shigella flexneri<\/jats:italic> 2a, <jats:italic>S. flexneri<\/jats:italic> 3a, <jats:italic>S. flexneri<\/jats:italic> 6, <jats:italic>Shigella boydii<\/jats:italic> 4, <jats:italic>and Shigella sonnei<\/jats:italic>) were examined in a guinea pig colitis model. A monovalent or hexavalent mixture containing 1\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> of each serogroup\/serotype of heat\u2010killed <jats:italic>Shigella<\/jats:italic> cells was administered orally on Days 0, 7, 14 and 21. On Day 28, the immunized animals were challenged rectally with 1\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> live virulent cells of each of the six <jats:italic>Shigella<\/jats:italic> serogroups\/serotypes. In all immunized groups, significant levels of protection were observed after these challenges. The serum titers of IgG and IgA against the lipopolysaccharide of each of the six <jats:italic>Shigella<\/jats:italic> serogroups\/serotypes increased exponential during the course of immunization. High IgA titers against the lipopolysaccharide of each of the six <jats:italic>Shigella<\/jats:italic> serogroups\/serotypes were also observed in intestinal lavage fluid from all immunized animals. These data indicate that a hexavalent mixture of heat\u2010killed cells of the six <jats:italic>Shigella<\/jats:italic> serogroups\/serotypes studied would be a possible broad\u2010spectrum candidate vaccine against shigellosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12095","type":"journal-article","created":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T13:27:22Z","timestamp":1378301242000},"page":"762-771","source":"Crossref","is-referenced-by-count":15,"title":["Protective immunity by oral immunization with heat\u2010killed <i>Shigella<\/i> strains in a guinea pig colitis model"],"prefix":"10.1111","volume":"57","author":[{"given":"Soumik","family":"Barman","sequence":"first","affiliation":[{"name":"National Institute of Cholera and Enteric Diseases P\u201033, C.I.T. Road, Scheme XM"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemanta","family":"Koley","sequence":"additional","affiliation":[{"name":"National Institute of Cholera and Enteric Diseases P\u201033, C.I.T. Road, Scheme XM"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thandavarayan","family":"Ramamurthy","sequence":"additional","affiliation":[{"name":"National Institute of Cholera and Enteric Diseases P\u201033, C.I.T. Road, Scheme XM"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoj Kumar","family":"Chakrabarti","sequence":"additional","affiliation":[{"name":"National Institute of Cholera and Enteric Diseases P\u201033, C.I.T. Road, Scheme XM"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumio","family":"Shinoda","sequence":"additional","affiliation":[{"name":"Collaborative Research Center of Okayama University for Infectious Diseases in India 67 Dr. S. C. 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