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For example, axin expression suppresses herpes simplex virus (HSV)\u2010induced necrotic cell death and enhances viral replication. Based on these observations, this study investigated the involvement of autophagy in regulation of HSV replication and found axin expression inhibits autophagy\u2010mediated suppression of viral replication in L929 cells. HSV infection induced autophagy in a time\u2010 and viral dose\u2010dependent manner in control L929 cells (L\u2010EV), whereas virus\u2010induced autophagy was delayed in axin\u2010expressing L929 cells (L\u2010axin). Subsequent analysis showed that induction of autophagy by rapamycin reduced HSV replication, and that inhibiting autophagy by 3\u2010methyladenine (3MA) and beclin\u20101 knockdown facilitated viral replication in L\u2010EV cells. In addition, preventing autophagy with 3MA suppressed virus\u2010induced cytotoxicity in L\u2010EV cells. In contrast, HSV replication in L\u2010axin cells was resistant to changes in autophagy. These results suggest that axin expression may render L929 cells resistant to HSV\u2010infection induced autophagy, leading to enhanced viral replication.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12123","type":"journal-article","created":{"date-parts":[[2013,12,11]],"date-time":"2013-12-11T14:06:03Z","timestamp":1386770763000},"page":"103-111","source":"Crossref","is-referenced-by-count":6,"title":["Axin expression delays herpes simplex virus\u2010induced autophagy and enhances viral replication in L929 cells"],"prefix":"10.1111","volume":"58","author":[{"given":"Eun\u2010Jin","family":"Choi","sequence":"first","affiliation":[{"name":"Laboratory of Cell Biology, Department of Microbiology and Bank for Pathogenic Virus, College of Medicine Korea University Seoul 136\u2010705 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun\u2010Ho","family":"Kee","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Department of Microbiology and 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