{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:43:15Z","timestamp":1793216595306,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,3,5]],"date-time":"2014-03-05T00:00:00Z","timestamp":1393977600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Development of complementary and\/or alternative drugs for treatment of hepatitis C virus (HCV) infection is still much needed from clinical and economic points of view. Antiviral substances obtained from medicinal plants are potentially good targets to study. <jats:italic>Glycyrrhiza uralensis<\/jats:italic> and <jats:italic> G. glabra<\/jats:italic> have been commonly used in both traditional and modern medicine. In this study, extracts of <jats:italic>G. uralensis<\/jats:italic> roots and their components were examined for anti\u2010HCV activity using an HCV cell culture system. It was found that a methanol extract of <jats:italic>G. uralensis<\/jats:italic> roots and its chloroform fraction possess anti\u2010HCV activity with 50%\u2010inhibitory concentrations (IC<jats:sub>50<\/jats:sub>) of 20.0 and 8.0\u2009\u03bcg\/mL, respectively. Through bioactivity\u2010guided purification and structural analysis, glycycoumarin, glycyrin, glycyrol and liquiritigenin were isolated and identified as anti\u2010HCV compounds, their IC<jats:sub>50<\/jats:sub> being 8.8, 7.2, 4.6 and 16.4\u2009\u03bcg\/mL, respectively. However, glycyrrhizin, the major constituent of <jats:italic>G. uralensis<\/jats:italic>, and its monoammonium salt, showed only marginal anti\u2010HCV activity. It was also found that licochalcone A and glabridin, known to be exclusive constituents of <jats:italic>G. inflata<\/jats:italic> and <jats:italic>G. glabra<\/jats:italic>, respectively, did have anti\u2010HCV activity, their IC<jats:sub>50<\/jats:sub> being 2.5 and 6.2\u2009\u03bcg\/mL, respectively. Another chalcone, isoliquiritigenin, also showed anti\u2010HCV activity, with an IC<jats:sub>50<\/jats:sub> of 3.7\u2009\u03bcg\/mL. Time\u2010of\u2010addition analysis revealed that all <jats:italic>Glycyrrhiza<\/jats:italic>\u2010derived anti\u2010HCV compounds tested in this study act at the post\u2010entry step. In conclusion, the present results suggest that glycycoumarin, glycyrin, glycyrol and liquiritigenin isolated from <jats:italic>G. uralensis,<\/jats:italic> as well as isoliquiritigenin, licochalcone A and glabridin, would be good candidates for seed compounds to develop antivirals against HCV.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12127","type":"journal-article","created":{"date-parts":[[2014,1,7]],"date-time":"2014-01-07T14:25:29Z","timestamp":1389104729000},"page":"180-187","source":"Crossref","is-referenced-by-count":134,"title":["Anti\u2010hepatitis C virus compounds obtained from <i>Glycyrrhiza uralensi<\/i>s and other <i>Glycyrrhiza<\/i> species"],"prefix":"10.1111","volume":"58","author":[{"given":"Myrna","family":"Adianti","sequence":"first","affiliation":[{"name":"Division of Microbiology Kobe University Graduate School of Medicine 7\u20105\u20101 Kusunoki\u2010cho Chuo\u2010ku Kobe 650\u20100017"},{"name":"Institute of Tropical Disease Airlangga University Jl. Mulyorejo Surabaya 60115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Aoki","sequence":"additional","affiliation":[{"name":"Division of Microbiology Kobe University Graduate School of Medicine 7\u20105\u20101 Kusunoki\u2010cho Chuo\u2010ku Kobe 650\u20100017"},{"name":"Japan Science and Technology\/Japan International Cooperation Agency Science and Technology Research Partnership for Sustainable Development Laboratory (JST\/JICA SATREPS), Faculty of Medicine University of Indonesia Jl. 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