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The hemolysin is secreted extracellularly as an inactive protoxin and converted to a mature toxin through removal of the N\u2010terminal propeptide, which comprises 151 amino acid residues. In this study, a novel protease having the trypsin\u2010like substrate specificity was purified from the bacterial culture supernatant. The N\u2010terminal amino acid sequence of the purified protein was identical with putative trypsin VMD27150 of <jats:italic>V. mimicus<\/jats:italic> strain VM573. The purified protease was found to cause maturation of the protoxin by cleavage of the Arg<jats:sup>151<\/jats:sup>\uf8ffSer<jats:sup>152<\/jats:sup> bond. Deletion of the protease gene resulted in increased amounts of the protoxin in the culture supernatant. In addition, expression of the hemolysin and protease genes was detected during the logarithmic growth phase. These findings indicate that the protease purified may mediate maturation of the hemolysin.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12177","type":"journal-article","created":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T17:13:25Z","timestamp":1405444405000},"page":"503-512","source":"Crossref","is-referenced-by-count":5,"title":["A novel extracellular protease of <i>Vibrio mimicus<\/i> that mediates maturation of an endogenous hemolysin"],"prefix":"10.1111","volume":"58","author":[{"given":"Tamaki","family":"Mizuno","sequence":"first","affiliation":[{"name":"Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University 1\u20101\u20101, Tsushima\u2010Naka Kita\u2010Ku Okayama 700\u20108530 Japan"},{"name":"Collaborative Research Center of Okayama University for Infectious Diseases in India National Institute of Cholera and Enteric Diseases 57 Dr. S. C. Banerjee Road, ID Hospital Campus, Beliaghata Kolkata West Bengal 700010 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Nanko","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University 1\u20101\u20101, Tsushima\u2010Naka Kita\u2010Ku Okayama 700\u20108530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Maehara","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University 1\u20101\u20101, Tsushima\u2010Naka Kita\u2010Ku Okayama 700\u20108530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumio","family":"Shinoda","sequence":"additional","affiliation":[{"name":"Collaborative Research Center of Okayama University for Infectious Diseases in India National Institute of Cholera and Enteric Diseases 57 Dr. S. C. Banerjee Road, ID Hospital Campus, Beliaghata Kolkata West Bengal 700010 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010Ichi","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University 1\u20101\u20101, Tsushima\u2010Naka Kita\u2010Ku Okayama 700\u20108530 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-99-2-169"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.1.3.245"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-40-3-194"},{"key":"e_1_2_8_5_1","first-page":"231","article-title":"Enteropathogenic factors produced by vibrios other than cholera toxin","volume":"9","author":"Shinoda S.","year":"2000","journal-title":"J Nat Toxins"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1248\/jhs.46.63"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2004.tb03551.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.5.1830-1835.1997"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2005.tb03631.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00809.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1248\/jhs.54.686"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1248\/jhs.53.430"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1997.tb01183.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06827.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.55.8.2073-2078.1989"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.12.4310-4317.1991"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1183-784"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)97200-5"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1985.tb00862.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1128\/iai.55.8.1936-1939.1987","article-title":"Activation of the plasma kallikrein\u2010kinin system by Vibrio vulnificus protease","volume":"55","author":"Miyoshi N.","year":"1987","journal-title":"Infect Immun"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2002.tb02699.x"},{"key":"e_1_2_8_22_1","first-page":"263","article-title":"Purification and characterization of a novel alkaline serine protease secreted by Vibrio metschnikovii","volume":"29","author":"Park J.Y.","year":"2011","journal-title":"Int J Mol Med"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-011-0969-y"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-9-258"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.211078"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12177","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:40:02Z","timestamp":1696466402000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":24,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1111\/1348-0421.12177"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12177","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9]]}}}