{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:28:05Z","timestamp":1792798085698,"version":"4.5.0"},"reference-count":23,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,11,2]],"date-time":"2014-11-02T00:00:00Z","timestamp":1414886400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Culture, Sport, Science and Technology","doi-asserted-by":"crossref","award":["Gunma University Operation Grants"],"award-info":[{"award-number":["Gunma University Operation Grants"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Japanese Ministry of Health, Labor and Welfare","award":["H24-Shinkou-Ippan-010"],"award-info":[{"award-number":["H24-Shinkou-Ippan-010"]}],"id":[{"id":"https:\/\/ror.org\/03mwa7s65","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Japanese Ministry of Health, Labor and Welfare","award":["H24-Shokuhin-Ippan-008"],"award-info":[{"award-number":["H24-Shokuhin-Ippan-008"]}],"id":[{"id":"https:\/\/ror.org\/03mwa7s65","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Compared with other developed countries, vancomycin\u2010resistant enterococci (VRE) are not widespread in clinical environments in Japan. There have been no VRE outbreaks and only a few VRE strains have sporadically been isolated in our university hospital in Gunma, Japan. To examine the drug susceptibility of\n                      <jats:italic>Enterococcus faecalis<\/jats:italic>\n                      and nosocomial infection caused by non\u2010VRE strains, a retrospective surveillance was conducted in our university hospital. Molecular epidemiological analyses were performed on 1711\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      clinical isolates collected in our hospital over a 6\u2010year period [1998\u20132003]. Of these isolates, 1241 (72.5%) were antibiotic resistant and 881 (51.5%) were resistant to two or more drugs. The incidence of multidrug resistant\n                      <jats:italic>E. faecalis<\/jats:italic>\n                      (MDR\u2010Ef) isolates in the intensive care unit increased after enlargement and restructuring of the hospital. The major group of MDR\u2010Ef strains consisted of 209 isolates (12.2%) resistant to the five drug combination tetracycline\/erythromycin\/kanamycin\/streptomycin\/gentamicin. Pulsed\u2010field gel electrophoresis analysis of the major MDR\u2010Ef isolates showed that nosocomial infections have been caused by MDR\u2010Ef over a long period (more than 3 years). Multilocus sequence typing showed that these strains were mainly grouped into ST16 (CC58) or ST64 (CC8). Mating experiments suggested that the drug resistances were encoded on two conjugative transposons (integrative conjugative elements), one encoded tetracycline\u2010resistance and the other erythromycin\/kanamycin\/streptomycin\/gentamicin\u2010resistance. To our knowledge, this is the first report of nosocomial infection caused by vancomycin\u2010susceptible MDR\u2010Ef strains over a long period in Japan.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1348-0421.12190","type":"journal-article","created":{"date-parts":[[2014,8,22]],"date-time":"2014-08-22T03:03:17Z","timestamp":1408676597000},"page":"607-614","source":"Crossref","is-referenced-by-count":16,"title":["Nosocomial infection caused by vancomycin\u2010susceptible multidrug\u2010resistant\n                    <i>Enterococcus faecalis<\/i>\n                    over a long period in a university hospital in Japan"],"prefix":"10.1111","volume":"58","author":[{"given":"Michiaki","family":"Kudo","sequence":"first","affiliation":[{"name":"Department of General Surgical Science (Surgery I)"},{"name":"Department of Bacteriology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Nomura","sequence":"additional","affiliation":[{"name":"Department of Bacteriology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachie","family":"Yomoda","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Clinical Laboratory Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Tanimoto","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Drug Resistance Gunma University Graduate School of Medicine Maebashi Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruyoshi","family":"Tomita","sequence":"additional","affiliation":[{"name":"Department of Bacteriology"},{"name":"Laboratory of Bacterial Drug Resistance Gunma University Graduate School of Medicine Maebashi Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03214.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555817923.ch10"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.30.9.2373-2378.1992"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/14.6.1173"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)91037-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","DOI":"10.2807\/ese.13.47.19046-en","article-title":"Emergence and spread of vancomycin resistance among enterococci in Europe","author":"Werner G.","year":"2008","journal-title":"Euro Surveill"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1128\/JCM.36.9.2460-2464.1998","article-title":"Evidence of nosocomial infection in Japan caused by high\u2010level gentamicin\u2010resistant Enterococcus faecalis  and identification of the pheromone\u2010responsive conjugative plasmid encoding gentamicin resistance","volume":"36","author":"Ma X.","year":"1998","journal-title":"J Clin Microbiol"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1128\/AAC.42.8.2150","article-title":"First report of the isolation of high\u2010level vancomycin\u2010resistant Enterococcus faecium  from a patient in Japan","volume":"42","author":"Fujita N.","year":"1998","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_2_8_10_1","unstructured":"Japan Nosocomial Infections Surveillance Releases and announcements. [Cited in March 2014] Available from URL:www.nih\u2010janis.jp\/english."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0606.000604"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1086\/313949"},{"key":"e_1_2_8_13_1","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1128\/AAC.41.3.702","article-title":"High\u2010level plasmid\u2010mediated gentamicin resistance and pheromone response of plasmids present in clinical isolates of Enterococcus faecalis","volume":"41","author":"Shiojima M","year":"1997","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.07.001"},{"key":"e_1_2_8_16_1","unstructured":"Multi Locus Sequence Typing Enterococcus faecalis [Cited in December 2013] Available from URL:http:\/\/efaecalis.mlst.net."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6430"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4161\/mge.1.1.15409"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)88930-1"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2382"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.12.6457-6461.2002"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/107662903764736346"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059491"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2009.0028"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12190","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12190","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T11:01:14Z","timestamp":1696503674000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11]]},"references-count":23,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1111\/1348-0421.12190"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12190","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11]]}}}