{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T11:25:18Z","timestamp":1784287518771,"version":"3.55.0"},"reference-count":13,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T00:00:00Z","timestamp":1419292800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Measles, mumps and rubella are vaccine\u2010preventable diseases; however limited epidemiological data are available from low\u2010income or developing countries. Thus, it is important to investigate the transmission of these viruses in different geographical regions. In this context, a cell culture\u2010based rapid and reliable immuno\u2010colorimetric assay (ICA) was established and its utility studied. Twenty\u2010three measles, six mumps and six rubella virus isolates and three vaccine strains were studied. Detection by ICA was compared with plaque and RT\u2010PCR assays. In addition, ICA was used to detect viruses in throat swabs (<jats:italic>n<\/jats:italic>\u2009=\u200924) collected from patients with suspected measles or mumps. Similarly, ICA was used in a focus reduction neutralization test (FRNT) and the results compared with those obtained by a commercial IgG enzyme immuno assay. Measles and mumps virus were detected 2 days post\u2010infection in Vero or Vero\u2010human signaling lymphocytic activation molecule cells, whereas rubella virus was detected 3 days post\u2010infection in Vero cells. The blue stained viral foci were visible by the naked eye or through a magnifying glass. In conclusion, ICA was successfully used on 35 virus isolates, three vaccine strains and clinical specimens collected from suspected cases of measles and mumps. Furthermore, an application of ICA in a neutralization test (i.e., FRNT) was documented; this may be useful for sero\u2010epidemiological, cross\u2010neutralization and pre\/post\u2010vaccine studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12201","type":"journal-article","created":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T17:59:19Z","timestamp":1411149559000},"page":"666-674","source":"Crossref","is-referenced-by-count":25,"title":["Detection of measles, mumps and rubella viruses by immuno\u2010colorimetric assay and its application in focus reduction neutralization tests"],"prefix":"10.1111","volume":"58","author":[{"given":"Sunil R.","family":"Vaidya","sequence":"first","affiliation":[{"name":"WHO Accredited National Measles Reference Laboratory National Institute of Virology (Indian Council of Medical Research) 20\u2010A Dr Ambedkar Road P.O. Box 11 Pune 411 001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelakshi S.","family":"Kumbhar","sequence":"additional","affiliation":[{"name":"WHO Accredited National Measles Reference Laboratory National Institute of Virology (Indian Council of Medical Research) 20\u2010A Dr Ambedkar Road P.O. Box 11 Pune 411 001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vandana S.","family":"Bhide","sequence":"additional","affiliation":[{"name":"WHO Accredited National Measles Reference Laboratory National Institute of Virology (Indian Council of Medical Research) 20\u2010A Dr Ambedkar Road P.O. Box 11 Pune 411 001 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,23]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"217","article-title":"Mumps virus nomenclature update: 2012","volume":"87","author":"World Health Organization","year":"2012","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_2_8_3_1","first-page":"390","article-title":"Indian Academy of Pediatrics. IAP committee on immunization","volume":"44","author":"Singhal T.","year":"2007","journal-title":"Indian Pediatr"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1985.tb00831.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(88)90092-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.08.021"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2011.08.026"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.08.081"},{"key":"e_1_2_8_9_1","unstructured":"World Health Organization. (2006) WHO Manual for the Laboratory Diagnosis of Measles and Rubella Virus Infection. [Cited 14 July 2006.] Available from URL:http:\/\/www.who.int\/immunization monitoring\/LabManualFinal.pdf."},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.9.4399-4401.2001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.09.006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-71-3-615"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.11.049"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0034-89102000000400007"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12201","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T16:36:30Z","timestamp":1696523790000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12]]},"references-count":13,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/1348-0421.12201"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12201","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12]]}}}