{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:59:45Z","timestamp":1788872385916,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T00:00:00Z","timestamp":1419292800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004396","name":"The Thailand Research Fund","doi-asserted-by":"crossref","award":["MRG5380022"],"award-info":[{"award-number":["MRG5380022"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Extended\u2010spectrum \u03b2\u2010lactamases (ESBLs) produced by\n                      <jats:italic>Enterobacteriaceae<\/jats:italic>\n                      are one of the resistance mechanisms to most \u03b2\u2010lactam antibiotics. ESBLs are currently a major problem in both hospitals and community settings worldwide. Rapid and reliable means of detecting ESBL\u2010producing bacteria is necessary for identification, prevention and treatment. Loop\u2010mediated isothermal amplification (LAMP) is a technique that rapidly amplifies DNA with high specificity and sensitivity under isothermal conditions. This study was aimed to develop a convenient, accurate and inexpensive method for detecting ESBL\u2010producing bacteria by a LAMP technique. ESBLs\u2010producing\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      and\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      were isolated from a tertiary hospital in Bangkok, Thailand and reconfirmed by double\u2010disk synergy test. A set of four specific oligonucleotide primers of LAMP for detection of\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX\u2010M9<\/jats:sub>\n                      gene was designed based on\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX\u2010M9<\/jats:sub>\n                      from\n                      <jats:italic>E. coli<\/jats:italic>\n                      (GenBank Accession No. AJ416345). The LAMP reaction was amplified under isothermal temperature at 63\u00b0C for 60\u2009min. Ladder\u2010like patterns of band sizes from 226 bp of the\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX\u2010M9<\/jats:sub>\n                      DNA target was observed. The LAMP product was further analyzed by restriction digestion with\n                      <jats:italic>Mbo<\/jats:italic>\n                      I and\n                      <jats:italic>Taq<\/jats:italic>\n                      I endonucleases. The fragments generated were approximately 168, 177 and 250 bp in size for\n                      <jats:italic>Mbo<\/jats:italic>\n                      I digestion and 165, 193, 229, 281 and 314 bp for\n                      <jats:italic>Taq<\/jats:italic>\n                      I digestion, which is in agreement with the predicted sizes. The sensitivity of the LAMP technique to\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX\u2010M9<\/jats:sub>\n                      was greater than that of the PCR method by at least 10,000\u2010fold. These results showed that the LAMP primers specifically amplified only the\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>CTX\u2010M9<\/jats:sub>\n                      gene. Moreover, the presence of LAMP amplicon was simply determined by adding SYBR Green I in the reaction. In conclusion, this technique for detection of ESBLs is convenient, reliable and easy to perform routinely in hospitals or laboratory units in developing countries.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1348-0421.12205","type":"journal-article","created":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T02:52:59Z","timestamp":1412563979000},"page":"655-665","source":"Crossref","is-referenced-by-count":11,"title":["Loop\u2010mediated isothermal amplification assay targeting the\n                    <i>bla<\/i>\n                    <sub>CTX\u2010M9<\/sub>\n                    gene for detection of extended spectrum \u03b2\u2010lactamase\u2010producing\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1111","volume":"58","author":[{"given":"Krit","family":"Thirapanmethee","sequence":"first","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanokporn","family":"Pothisamutyothin","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surakit","family":"Nathisuwan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Pharmacy Mahidol University 447 Sri Ayudthaya Road Rajthevi Bangkok 10400 Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mullika T.","family":"Chomnawang","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanpen","family":"Wiwat","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2165\/11533040-000000000-00000"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.10.2856-2861.2001"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-777X.68531"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3343\/kjlm.2008.28.6.401"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2012.691460"},{"key":"e_1_2_8_9_1","unstructured":"Lahey Clinic.OXA\u2010type \u03b2\u2010lactamases. 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