{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:53:13Z","timestamp":1782921193223,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,1,18]],"date-time":"2015-01-18T00:00:00Z","timestamp":1421539200000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004595","name":"Universiti Sains Malaysia","doi-asserted-by":"crossref","award":["001\/PSKBP\/8630015"],"award-info":[{"award-number":["001\/PSKBP\/8630015"]}],"id":[{"id":"10.13039\/501100004595","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004595","name":"Universiti Sains Malaysia","doi-asserted-by":"crossref","award":["001\/PSKBP\/8630011"],"award-info":[{"award-number":["001\/PSKBP\/8630011"]}],"id":[{"id":"10.13039\/501100004595","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>The detection and measurement of different antibody isotypes in the serum provide valuable indicators of the different stages of typhoid infection. Here, the ability of S. Typhi recombinant hemolysin E (HlyE) to detect multi\u2010isotype antibody responses in sera of patients with typhoid and paratyphoid A was investigated using an indirect antibody immunoassay. Nanogram amounts of HlyE were found to be sufficient for detection of IgG and IgA isotypes and, in a study of individuals' sera (n\u2009=\u2009100), the immunoassay was able to distinguish between typhoid and non\u2010typhoid sera. The overall sensitivity, specificity and efficiency of the ELISA were 70% (39\/56), 100% (44\/44) and 83% respectively.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12211","type":"journal-article","created":{"date-parts":[[2014,11,15]],"date-time":"2014-11-15T01:49:57Z","timestamp":1416016197000},"page":"43-47","source":"Crossref","is-referenced-by-count":7,"title":["Multi\u2010isotype antibody responses against the multimeric <i>Salmonella<\/i> Typhi recombinant hemolysin E antigen"],"prefix":"10.1111","volume":"59","author":[{"given":"Eugene Boon Beng","family":"Ong","sequence":"first","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia 11800 Minden Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Ignatius","sequence":"additional","affiliation":[{"name":"School of Mathematical Sciences Universiti Sains Malaysia  11800 Minden Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy Amilda","family":"Anthony","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia 11800 Minden Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Aziah","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia 11800 Minden Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Ismail","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia 11800 Minden Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theam Soon","family":"Lim","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia 11800 Minden Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,1,18]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.05.010"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis024"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01435.x"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00779-06"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00104-10"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep01043"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08026"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-254-4"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-29-3-195"},{"key":"e_1_2_4_11_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1128\/iai.52.1.209-212.1986","article-title":"Antibodies to porin antigens of Salmonella typhi induced during typhoid infection in humans","volume":"52","author":"Calderon I.","year":"1986","journal-title":"Infect Immun"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.11.087627"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00661-13"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2008.06.004"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2005.12.011"},{"key":"e_1_2_4_16_1","doi-asserted-by":"crossref","first-page":"20594","DOI":"10.2807\/1560-7917.ES2013.18.39.20594","article-title":"Unusual increase in reported cases of paratyphoid A fever among travellers returning from Cambodia, January to September 2013","volume":"18","author":"Tourdjman M.","year":"2013","journal-title":"Euro Surveill"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit723"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12211","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T12:50:56Z","timestamp":1696510256000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1111\/1348-0421.12211"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12211","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1]]}}}