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However, DNA vaccines usually induce weak immune responses. In this study, it was found that the efficacy of a DNA vaccine expressing Cap protein was improved by simultaneous expression of porcine IL\u20106. A plasmid (pIRES\u2010ORF2\/IL6) separately expressing both Cap protein and porcine IL\u20106 was constructed and compared with another plasmid (pIRES\u2010ORF2) expressing Cap protein for its potential to induce PCV2\u2010specific immune responses. Mice were vaccinated i.m. twice at 3 week intervals and the induced humoral and cellular responses evaluated. All animals vaccinated with pIRES\u2010ORF2\/IL6 and pIRES\u2010ORF2 developed specific anti\u2010PCV2 antibodies (according to enzyme\u2010linked immunosorbent assay) and a T lymphocyte proliferation response. The percentages of CD3<jats:sup>+<\/jats:sup>, CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>, and CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup> subgroups of peripheral blood T\u2010lymphocytes were significantly higher in mice immunized with pIRES\u2010ORF2\/IL6 than in those that had received pIRES\u2010ORF2. After challenge with the virulent PCV2 Wuzhi isolate, mice vaccinated with pIRES\u2010ORF2\/IL6 had significantly less viral replication than those vaccinated with pIRES\u2010ORF2, suggesting that the protective immunity induced by pIRES\u2010ORF2\/IL6 is superior to that induced by pIRES\u2010ORF2.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12244","type":"journal-article","created":{"date-parts":[[2015,2,9]],"date-time":"2015-02-09T10:58:07Z","timestamp":1423479487000},"page":"174-180","source":"Crossref","is-referenced-by-count":15,"title":["Enhancement of the immunogenicity of a porcine circovirus type 2 DNA vaccine by a recombinant plasmid coexpressing capsid protein and porcine interleukin\u20106 in mice"],"prefix":"10.1111","volume":"59","author":[{"given":"Xiao\u2010Qing","family":"Guo","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Henan Agricultural University 63 Nongye Road Zhengzhou 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