{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T04:55:35Z","timestamp":1771649735680,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grants-in-Aid for Scientific Research B","award":["26293439"],"award-info":[{"award-number":["26293439"]}]},{"name":"Young Scientists (B)","award":["25861783"],"award-info":[{"award-number":["25861783"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Both <jats:italic>Streptococcus<\/jats:italic> and <jats:italic>Actinomyces<\/jats:italic> can produce acids from dietary sugars and are frequently found in caries lesions. In the oral cavity, nitrogenous compounds, such as peptides and amino acids, are provided continuously by saliva and crevicular gingival fluid. Given that these bacteria can also utilize nitrogen compounds for their growth, it was hypothesized that nitrogenous compounds may influence their acid production; however, no previous studies have examined this topic. Therefore, the present study aimed to assess the effects of nitrogenous compounds (tryptone and glutamate) on glucose\u2010derived acid production by <jats:italic>Streptococcus<\/jats:italic> and <jats:italic>Actinomyces<\/jats:italic>. Acid production was evaluated using a pH\u2010stat method under anaerobic conditions, whereas the amounts of metabolic end\u2010products were quantified using high performance liquid chromatography. Tryptone enhanced glucose\u2010derived acid production by up to 2.68\u2010fold, whereas glutamate enhanced <jats:italic>Streptococcus<\/jats:italic> species only. However, neither tryptone nor glutamate altered the end\u2010product profiles, indicating that the nitrogenous compounds stimulate the whole metabolic pathways involving in acid production from glucose, but are not actively metabolized, nor do they alter metabolic pathways. These results suggest that nitrogenous compounds in the oral cavity promote acid production by <jats:italic>Streptococcus<\/jats:italic> and <jats:italic>Actinomyces in vivo<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12283","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T16:09:36Z","timestamp":1436890176000},"page":"501-506","source":"Crossref","is-referenced-by-count":16,"title":["Nitrogenous compounds stimulate glucose\u2010derived acid production by oral <i>Streptococcus<\/i> and <i>Actinomyces<\/i>"],"prefix":"10.1111","volume":"59","author":[{"given":"Yuka","family":"Norimatsu","sequence":"first","affiliation":[{"name":"Division of Orthodontics and Dentofacial Orthopedics"},{"name":"Division of Oral Ecology and Biochemistry Tohoku University Graduate School of Dentistry 4\u20101 Seiryo\u2010machi Aoba\u2010ku Sendai 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