{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:39:53Z","timestamp":1787013593614,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National High Technology Research and Development Program of China","award":["2011AA10A210"],"award-info":[{"award-number":["2011AA10A210"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      poses a major threat to human health and to the mink industry. Thus, development of vaccines that elicit robust humoral and cellular immunity against\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      is greatly needed. In this study, a recombinant attenuated\n                      <jats:italic>Salmonella<\/jats:italic>\n                      vaccine (RASV) that expresses the outer membrane proteins fusion OprF\n                      <jats:sub>190\u2013342<\/jats:sub>\n                      \u2010OprI\n                      <jats:sub>21\u201383<\/jats:sub>\n                      (F1I2) from\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      was constructed and the potency of this vaccine candidate assessed by measuring F1I2\u2010specific humoral immune responses upon vaccination through s.c. or oral routes. S.C. administration achieved higher serum IgG titers and IgA titers in the intestine and induced stronger F1I2\u2010specific IgG and IgA titers in lung homogenate than did oral administration, which resulted in low IgG titers and no local IgA production. High titers of IFN\u2010\u03b3, IL\u20104, and T\u2010lymphocyte subsets induced a mixed Th1\/Th2 response in mice immunized s.c., indicating elicitation of cellular immunity. Importantly, when immunized mice were challenged with\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      by the intranasal route 30 days after the initial immunization, s.c. vaccination achieved 77.78% protection, in contrast to 41.18% via oral administration and 66.67% via\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      \u2010expressed F1I2 (His\u2010F1I2) vaccination. These results indicate that s.c. vaccination provides a better protective response against\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      infection than do oral administration and the His\u2010F1I2 vaccine.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1348-0421.12291","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T02:42:52Z","timestamp":1438915372000},"page":"533-544","source":"Crossref","is-referenced-by-count":16,"title":["<i>Salmonella<\/i>\n                    Typhimurium strain expressing OprF\u2010OprI protects mice against fatal infection by\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1111","volume":"59","author":[{"given":"Mingliang","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjiang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingmin","family":"Gu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwu","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Animal Science Jilin University No. 5333, Xi'an Street Changchun Jilin, 130062"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyin","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyu","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liancheng","family":"Lei","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyu","family":"Han","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine"},{"name":"Jiangsu Co\u2010innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou 225009 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,9]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","first-page":"S13","article-title":"Clinical features of Pseudomonas aeruginosa infections","volume":"13","author":"Sarlangue J.","year":"2006","journal-title":"Arch Pediatr"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.02.025"},{"key":"e_1_2_8_4_1","first-page":"378","article-title":"An outbreak of haemorrhagic pneumonia in mink","volume":"48","author":"Farrel R.","year":"1958","journal-title":"Cornell Vet"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-6147-z"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.11.029"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)00166-X"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2014.03.015"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00851-06"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2005.09.012"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1213"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.08.031"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"830","DOI":"10.4049\/jimmunol.101.5.830","article-title":"Delayed hypersensitivity and arthus reactivity in relation to host resistance in salmonella\u2010infected mice","volume":"101","author":"Collins F.M.","year":"1968","journal-title":"J Immunol"},{"key":"e_1_2_8_15_1","volume-title":"Selected Methods in Cellular Immunology","author":"Mishell B.B.","year":"1980"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00434-X"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.7.10.16369"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5376.565"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(06)91001-3"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00176-7"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108102"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820138909112265"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.1739-1749.2002"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.11.6546-6553.2004"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1128\/IAI.67.5.2145-2152.1999","article-title":"Immunogenicity of a Salmonella typhimurium aroA aroD vaccine expressing a nontoxic domain of Clostridium difficile toxin A","volume":"67","author":"Ward S.J.","year":"1999","journal-title":"Infect Immun"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2010.12.014"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(92)90065-V"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01495-07"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/bulm.1998.0074"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1998.tb00717.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008644"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.4049\/jimmunol.145.7.2281","article-title":"Oral Salmonella typhimurium (AroA\u2010) vaccine expressing a major leishmanial surface protein (gp63) preferentially induces T helper 1 cells and protective immunity against leishmaniasis","volume":"145","author":"Yang D.M.","year":"1990","journal-title":"J Immunol"},{"key":"e_1_2_8_33_1","first-page":"362","article-title":"A role for CD4+ T cells from orally immunized rats in enhanced clearance of Pseudomonas aeruginosa from the lung","volume":"83","author":"Dunkley M.","year":"1994","journal-title":"Immunology"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2012.09.006"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(02)00002-8"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2007.03.006"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.1739-1749.2002"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00457-07"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/14.2.111"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00093-08"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.1.199-203.2002"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0688-693"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)00173-X"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12291","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T18:36:22Z","timestamp":1696444582000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":42,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1111\/1348-0421.12291"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12291","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9]]}}}