{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:53:51Z","timestamp":1790751231583,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,11,24]],"date-time":"2015-11-24T00:00:00Z","timestamp":1448323200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic> is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used. Biofilm formation is believed to be one of the possible causes of relapse because of its ability to increase drug resistance. EPS in biofilms have been reported to be related to the limitation of antibiotic penetration in <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic>. However, the mechanisms by which biofilms restrict the diffusion of antibiotics remain unclear. The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in <jats:italic>B. pseudomallei<\/jats:italic> using <jats:italic>bps<\/jats:italic>I, <jats:italic>ppk<\/jats:italic>, and <jats:italic>rpo<\/jats:italic>S mutant strains. CLSM revealed a reduction in exopolysaccharide production and disabled micro\u2010colony formation in <jats:italic>B. pseudomallei<\/jats:italic> mutants, which paralleled the antibiotic resistance. Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the <jats:italic>bps<\/jats:italic>I mutant. These results indicate that the correlation between these phenomena in the <jats:italic>B. pseudomallei<\/jats:italic> biofilm at least results from the exopolysaccharide, which may be under the regulation of <jats:italic>bps<\/jats:italic>I, <jats:italic>ppk<\/jats:italic>, or <jats:italic>rpo<\/jats:italic>S genes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12331","type":"journal-article","created":{"date-parts":[[2015,10,21]],"date-time":"2015-10-21T09:22:57Z","timestamp":1445419377000},"page":"653-663","source":"Crossref","is-referenced-by-count":41,"title":["Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in <i>Burkholderia pseudomallei<\/i><i>bps<\/i>I, <i>ppk<\/i>, and <i>rpo<\/i>S mutant strains"],"prefix":"10.1111","volume":"59","author":[{"given":"Rungrawee","family":"Mongkolrob","sequence":"first","affiliation":[{"name":"Biochemistry Department, Faculty of Science Mahidol University Rama VI Road Bangkok 10400 Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwimol","family":"Taweechaisupapong","sequence":"additional","affiliation":[{"name":"Melioidosis Research Center and Biofilm research group, Faculty of Dentistry Khon Kaen University Khon Kaen 40002 Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumalee","family":"Tungpradabkul","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Faculty of Science Mahidol University Rama VI Road Bangkok 10400 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