{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:41:10Z","timestamp":1789472470384,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,2,19]],"date-time":"2016-02-19T00:00:00Z","timestamp":1455840000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>This study aimed to investigate the antifungal activity of <jats:italic>Rubus chingii<\/jats:italic> extract in combination with fluconazole (FLC) against FLC\u2010resistant <jats:italic>Candida albicans<\/jats:italic> 100 <jats:italic>in vitro<\/jats:italic>. A <jats:italic>R. chingii<\/jats:italic> extract and FLC\u2010resistant <jats:italic>C. albicans<\/jats:italic> fungus suspension were prepared. The minimum inhibitory concentration and fractional inhibitory concentration index of <jats:italic>R. chingii<\/jats:italic> extract combined with FLC against <jats:italic>C. albicans<\/jats:italic> were determined, after which growth curves for <jats:italic>C. albicans<\/jats:italic> treated with <jats:italic>R. chingii<\/jats:italic> extract, FLC alone and a combination of these preparations were constructed. Additionally, the mechanisms of drug combination against <jats:italic>C. albicans<\/jats:italic> were explored by flow cytometry, gas chromatographic mass spectrometry and drug efflux pump function detection. <jats:italic>R. chingii<\/jats:italic> extract combined with FLC showed significant synergy. Flow cytometry suggested that <jats:italic>C. albicans<\/jats:italic> cells mainly arrest in G1 and S phases when they have been treated with the drug combination. The drug combination resulted in a marked decrease in the ergosterol content of the cell membrane. Additionally, efflux of Rhodamine 6G decreased with increasing concentrations of <jats:italic>R. chingii<\/jats:italic> extract. <jats:italic>R. chingii<\/jats:italic> extract combined with FLC has antifungal activity against FLC\u2010resistant <jats:italic>C. albicans<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12357","type":"journal-article","created":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T16:28:18Z","timestamp":1452788898000},"page":"82-92","source":"Crossref","is-referenced-by-count":28,"title":["Antifungal activity of <i>Rubus chingii<\/i> extract combined with fluconazole against fluconazole\u2010resistant <i>Candida albicans<\/i>"],"prefix":"10.1111","volume":"60","author":[{"given":"Bing","family":"Han","sequence":"first","affiliation":[{"name":"Department of Pharmacy Minhang Hospital Fudan University 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