{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T17:24:32Z","timestamp":1758475472911,"version":"3.37.3"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T00:00:00Z","timestamp":1458086400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004482","name":"Kuwait University Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation and cell surface hydrophobicity (CSH) are all virulence traits involved in the pathogenicity of <jats:italic>Candida<\/jats:italic>. Post\u2010antifungal effect (PAFE) also have a bearing on pathogenicity and virulence of <jats:italic>Candida<\/jats:italic>. <jats:italic>Candida dubliniensis<\/jats:italic> is associated with oral and systemic candidosis, which can be managed with caspofungin. There is no published information on caspofungin\u2010induced PAFE and its impact on adhesion traits of <jats:italic>C. dubliniensis<\/jats:italic> isolates. Thus, the purpose of this investigation was to determine the <jats:italic>in vitro<\/jats:italic> duration of PAFE on 20 <jats:italic>C. dubliniensis<\/jats:italic> isolates following transient exposure to caspofungin. Furthermore the impacts of caspofungin\u2010induced PAFE on adhesion to BEC and DAS, GT formation and CSH of these isolates were also determined. After establishing the minimum inhibitory concentration (MIC) of caspofungin, <jats:italic>C. dubliniensis<\/jats:italic> isolates were exposed to sub\u2010lethal concentrations (\u00d73\u2009MIC) of caspofungin for 1\u2009hr. Thereafter the duration of PAFE, adhesion to BEC and DAS, GT formation and CSH were determined by previously described <jats:italic>in\u2010vitro<\/jats:italic> assays. MIC (\u03bcg\/mL) of <jats:italic>C. dubliniensis<\/jats:italic> isolates to caspofungin ranged from 0.004 to 0.19. Caspofungin\u2010induced mean PAFE on <jats:italic>C. dubliniensis<\/jats:italic> isolates was 2.17\u2009hr. Exposure to caspofungin suppressed the ability of <jats:italic>C. dubliniensis<\/jats:italic> isolates to adhere to BEC and DAS, form GT and CSH by 69.97%, 71.95%, 90.06% and 32.29% (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001 for all), respectively. Thus, transient exposure of <jats:italic>C. dubliniensis<\/jats:italic> isolates to caspofungin produces an antifungal effect not only by suppressing its growth but also by altering its adhesion traits.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12362","type":"journal-article","created":{"date-parts":[[2016,2,6]],"date-time":"2016-02-06T07:00:04Z","timestamp":1454742004000},"page":"160-167","source":"Crossref","is-referenced-by-count":4,"title":["Caspofungin\u2010induced <i>in\u2010vitro<\/i> post\u2010antifungal effect and its impact on adhesion related traits of oral <i>Candida dubliniensis<\/i> and <i>Candida albicans<\/i> isolates"],"prefix":"10.1111","volume":"60","author":[{"given":"Arjuna Nishantha Bandara","family":"Ellepola","sequence":"first","affiliation":[{"name":"Department of Bioclinical Sciences Faculty of Dentistry"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel","family":"Chandy","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Health Sciences Center, Kuwait University Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zia Uddin","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Health Sciences Center, Kuwait University Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshman Perera","family":"Samaranayake","sequence":"additional","affiliation":[{"name":"School of Dentistry University of Queensland Brisbane 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