{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T12:13:06Z","timestamp":1762603986723},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T00:00:00Z","timestamp":1460678400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Research Committee of Prion Disease and Slow Virus Infection"},{"name":"The Ministry of Health"},{"name":"Labor and Welfare of Japan"},{"name":"The High-Tech Center"},{"name":"JSPS KAKENHI","award":["26461286"],"award-info":[{"award-number":["26461286"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>JC polyomavirus (JCPyV) causes progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease of the central nervous system, in immunocompromised patients. Because no drugs have been approved for treating PML, many antiviral agents are currently being investigated for this purpose. The inhibitory effects of the topoisomerase I inhibitors topotecan and \u03b2\u2010lapachone were assessed by investigating viral replication, propagation and viral protein 1 (VP1) production in cultured cells. JCPyV replication was assayed using the human neuroblastoma cell line IMR\u201032 transfected with the JCPyV plasmid and RT\u2010 PCR combined with <jats:italic>Dpn<\/jats:italic> I treatment. <jats:italic>Dpn<\/jats:italic> I digests the input plasmid DNA containing methylated adenosine, but not newly replicated JCPyV DNA, in IMR\u201032 cells. It was found that JCPyV replicates less in IMR\u201032 cells treated with topotecan or \u03b2\u2010lapachone than in untreated cells. Moreover, drug treatment of JCI cells, which are IMR\u201032 cells persistently infected with JCPyV, led to a reduction in the amount of JCPyV DNA and population of VP1\u2010positive cells. These results demonstrate that topotecan and \u03b2\u2010lapachone affects JCPyV propagation in human neuroblastoma cell lines, suggesting that topotecan and \u03b2\u2010lapachone could potentially be used to treat PML.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12372","type":"journal-article","created":{"date-parts":[[2016,3,3]],"date-time":"2016-03-03T13:20:01Z","timestamp":1457011201000},"page":"253-260","source":"Crossref","is-referenced-by-count":13,"title":["Suppressive effect of topoisomerase inhibitors on JC polyomavirus propagation in human neuroblastoma cells"],"prefix":"10.1111","volume":"60","author":[{"given":"Souichi","family":"Nukuzuma","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Kobe Institute of Health 4\u20106\u20105, Minatojima\u2010Nakamachi Chuo\u2010ku, Kobe 650\u20100046"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Nakamichi","sequence":"additional","affiliation":[{"name":"Department of 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