{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T19:03:48Z","timestamp":1761419028452},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,5,18]],"date-time":"2016-05-18T00:00:00Z","timestamp":1463529600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>An ELISA that measures anti\u2010PT IgG antibody has been used widely for the serodiagnosis of pertussis; however, the IgG\u2010based ELISA is inadequate for patients during the acute phase of the disease because of the slow response of anti\u2010PT IgG antibodies. To solve this problem, we developed a novel IgM\u2010capture ELISA that measures serum anti\u2010<jats:italic>Bordetella pertussis<\/jats:italic> Vag8 IgM levels for the accurate and early diagnosis of pertussis. First, we confirmed that Vag8 was highly expressed in all B. pertussis isolates tested (<jats:italic>n<\/jats:italic>\u2009=\u200930), but little or none in other <jats:italic>Bordetella<\/jats:italic> species, and that DTaP vaccines did not induce anti\u2010Vag8 IgG antibodies in mice (i.e. the antibody level could be unaffected by the vaccination). To determine the immune response to Vag8 in <jats:italic>B. pertussis<\/jats:italic> infection, anti\u2010Vag8 IgM levels were compared between 38 patients (acute phase of pertussis) and 29 healthy individuals using the anti\u2010Vag8 IgM\u2010capture ELISA. The results revealed that the anti\u2010Vag8 IgM levels were significantly higher in the patients compared with the healthy individuals (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). ROC analysis also showed that the anti\u2010Vag8 IgM\u2010capture ELISA has higher diagnostic accuracy (AUC, 0.92) than a commercial anti\u2010PT IgG ELISA kit. Moreover, it was shown that anti\u2010Vag8 IgM antibodies were induced earlier than anti\u2010PT IgG antibodies on sequential patients\u2019 sera. These data indicate that our novel anti\u2010Vag8 IgM\u2010capture ELISA is a potentially useful tool for making the accurate and early diagnosis of <jats:italic>B. pertussis<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12378","type":"journal-article","created":{"date-parts":[[2016,3,21]],"date-time":"2016-03-21T06:12:45Z","timestamp":1458540765000},"page":"326-333","source":"Crossref","is-referenced-by-count":8,"title":["A Novel IgM\u2010capture enzyme\u2010linked immunosorbent assay using recombinant Vag8 fusion protein for the accurate and early diagnosis of <i>Bordetella pertussis<\/i> infection"],"prefix":"10.1111","volume":"60","author":[{"given":"Nao","family":"Otsuka","sequence":"first","affiliation":[{"name":"Department of Bacteriology II National Institute of Infectious Diseases Tokyo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kensei","family":"Gotoh","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Konan Kosei Hospital Konan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Konan Kosei Hospital Konan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Ozaki","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Konan Kosei Hospital Konan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukitsugu","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II National Institute of Infectious Diseases Tokyo"},{"name":"Department of Pediatrics St. Marianna University School of Medicine Kawasaki"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyohito","family":"Haga","sequence":"additional","affiliation":[{"name":"Denka Seiken Co. 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