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In this study, the antigenic epitopes of the gene encoding the 55\u2009kDa antigen fragment of <jats:italic>Pneumocystis<\/jats:italic> (p55), which may play an important role in <jats:italic>Pneumocystis<\/jats:italic> pneumonia, were analyzed. A gene containing tandem variants of the p55 antigen was synthesized and named the tandem antigen gene (TAG). TAG's potential as a DNA vaccine was assessed in immunosuppressed rats. Immunization with p55\u2010TAG DNA vaccine significantly reduced both the pathogen burden and lung\u2013weight to body\u2013weight ratios. 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