{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:00:30Z","timestamp":1762272030308},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,7,13]],"date-time":"2016-07-13T00:00:00Z","timestamp":1468368000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p>Eye spray influenza vaccines for chickens are increasingly available; however, how to enhance cellular and antibody responses to them remains undetermined. Here, eye\u2010drops containing the immune\u2010enhancing adjuvants Pam2CSK4 or polyI:C were assessed in chickens. Application of these TLR agonists to chicken conjunctiva resulted in up\u2010regulation of IL\u20101\u03b2, but not other cytokines, including IFN and IL\u20106, in the spleen, lung and Harderian gland. Thus, responses to adjuvant applied to the conjunctival mucosa of chickens differ from those expected from the responses to intra\u2010nasal adjuvants in mammals. Identifying an appropriate delivery route for adjuvants is crucial for evoking immune responses in chickens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12391","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T07:56:46Z","timestamp":1464681406000},"page":"511-515","source":"Crossref","is-referenced-by-count":3,"title":["Cytokine responses to eye spray adjuvants for enhancing vaccine\u2010induced immunity in chickens"],"prefix":"10.1111","volume":"60","author":[{"given":"Hiromi","family":"Takaki","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Hokkaido University Graduate School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruko","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Hokkaido University Graduate School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riho","family":"Kurata","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health National Agriculture and Food Research Organization (NARO) Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Hikono","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health National Agriculture and Food Research Organization (NARO) Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Hiono","sequence":"additional","affiliation":[{"name":"Department of Disease Control Hokkaido University Graduate School of Veterinary Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kida","sequence":"additional","affiliation":[{"name":"Research Center for Zoonosis Control Hokkaido University Kita\u2010ku Sapporo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Misako","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Hokkaido University Graduate School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiko","family":"Saito","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health National Agriculture and Food Research Organization (NARO) Tsukuba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsukasa","family":"Seya","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Hokkaido University Graduate School of Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2005.02.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2012.10.005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11523-012-0213-1"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2007.05.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.03.008"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12099"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012550"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7280"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.03.008"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12322"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2012.05.003"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105713"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000351583"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2007.08.004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103902200"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.01.048"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920208786241234"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.05.006"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.06.002"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.6.4354"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001755107"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.12.014"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2010.11.012"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2014.05.001"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12391","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12391","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T17:20:32Z","timestamp":1696526432000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":24,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1111\/1348-0421.12391"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12391","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7]]}}}