{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:04:36Z","timestamp":1791576276623,"version":"4.3.4"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,8,20]],"date-time":"2016-08-20T00:00:00Z","timestamp":1471651200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Japanese Ministry of Education, Culture, Sports, Science, and Technology Grant-in-Aid","award":["15H05935"],"award-info":[{"award-number":["15H05935"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Seborrheic dermatitis (SD) is a chronic inflammatory dermatologic condition in which erythema and itching develop on areas of the body with sebaceous glands, such as the scalp, face and chest. The inflammation is evoked directly by oleic acid, which is hydrolyzed from sebum by lipases secreted by skin microorganisms. Although the skin fungal genus, <jats:italic>Malassezia<\/jats:italic>, is thought to be the causative agent of SD, analysis of the bacterial microbiota of skin samples of patients with SD is necessary to clarify any association with <jats:italic>Malassezia<\/jats:italic> because the skin microbiota comprises diverse bacterial and fungal genera. In the present study, bacterial microbiotas were analyzed at non\u2010lesional and lesional sites of 24 patients with SD by pyrosequencing and qPCR. Principal coordinate analysis revealed clear separation between the microbiota of non\u2010lesional and lesional sites. <jats:italic>Acinetobacter<\/jats:italic>, <jats:italic>Corynebacterium<\/jats:italic>, <jats:italic>Staphylococcus<\/jats:italic>, <jats:italic>Streptococcus<\/jats:italic> and <jats:italic>Propionibacterium<\/jats:italic> were abundant at both sites. <jats:italic>Propionibacterium<\/jats:italic> was abundant at non\u2010lesional sites, whereas <jats:italic>Acinetobacter<\/jats:italic>, <jats:italic>Staphylococcus<\/jats:italic> and <jats:italic>Streptococcus<\/jats:italic> predominated at lesional sites; however, the extent of <jats:italic>Propionibacterium<\/jats:italic> colonization did not differ significantly between lesional and non\u2010lesional sites according to qPCR. Given that these abundant bacteria hydrolyze sebum, they may also contribute to SD development. To the best of our knowledge, this is the first comprehensive analysis of the bacterial microbiotas of the skin of SD patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12398","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T17:17:22Z","timestamp":1466011042000},"page":"521-526","source":"Crossref","is-referenced-by-count":78,"title":["Comprehensive pyrosequencing analysis of the bacterial microbiota of the skin of patients with seborrheic dermatitis"],"prefix":"10.1111","volume":"60","author":[{"given":"Akiomi","family":"Tanaka","sequence":"first","affiliation":[{"name":"Department of Microbiology Meiji Pharmaceutical University 2\u2010522\u20101 Noshio Kiyose Tokyo 204\u20108588"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Otomi","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Microbiology Meiji Pharmaceutical University 2\u2010522\u20101 Noshio Kiyose Tokyo 204\u20108588"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Microbiology Meiji Pharmaceutical University 2\u2010522\u20101 Noshio Kiyose Tokyo 204\u20108588"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mami","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Dermatology Tokyo Medical University 6\u20107\u20101 Nishi\u2010Shinjuku Tokyo 160\u20100023 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoji","family":"Tsuboi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Tokyo Medical University 6\u20107\u20101 Nishi\u2010Shinjuku Tokyo 160\u20100023 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Sugita","sequence":"additional","affiliation":[{"name":"Department of Microbiology Meiji Pharmaceutical University 2\u2010522\u20101 Noshio Kiyose Tokyo 204\u20108588"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1382"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2013.01.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.5021\/ad.2011.23.S3.S350"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10570.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2003.12.034"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.8.9.1365"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000051628"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jidsymp.5650049"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1087-0024.2005.10119.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701017"},{"key":"e_1_2_8_12_1","first-page":"239","article-title":"Molecular characterization of the skin fungal microbiota in patients with seborrheic dermatitis","volume":"5","author":"Tanaka A.","year":"2014","journal-title":"J Clin Exp Dermatol Res"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-007-9320-4"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00597-10"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1382"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058203"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12684"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2537"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12398","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12398","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T16:59:06Z","timestamp":1696525146000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12398"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8]]},"references-count":18,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["10.1111\/1348-0421.12398"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12398","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8]]}}}