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However, whether the novel Ig isotype IgZ is present in the Indian major carp, <jats:italic>Catla catla,<\/jats:italic> has not yet been elucidated. The present study reports the presence of IgZ ortholog in <jats:italic>C. catla<\/jats:italic> (<jats:italic>Cc<\/jats:italic>IgZ) and further demonstrates its comparative tissue specific expression with IgM (<jats:italic>Cc<\/jats:italic>IgM) in response to bacterial and parasitic stimulation. The putative 139 amino acid sequence of IgZ heavy chain cDNA of <jats:italic>C. catla<\/jats:italic> showed homology with IgZ constant domains of other teleosts. Phylogenetic analysis of the predicted IgZ transcript sequence clustered with previously identified IgZ heavy chain sequences of <jats:italic>Cyprinidae<\/jats:italic> family members. The inductive expression profiles of IgZ and IgM genes were evaluated in immunologically relevant tissues at 24, 48 and 72 hr post infection with <jats:italic>Aeromonas hydrophila<\/jats:italic>, <jats:italic>Streptococcus uberis<\/jats:italic> and <jats:italic>Argulus<\/jats:italic> sp. Both <jats:italic>Cc<\/jats:italic>IgZ and <jats:italic>Cc<\/jats:italic>IgM were expressed most strongly in the kidneys of healthy fish. Basal expression of <jats:italic>Cc<\/jats:italic>IgM transcript was higher than that of <jats:italic>Cc<\/jats:italic>IgZ in all the examined tissues. Stimulation with bacteria triggered significant increase of IgZ in the intestine (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and spleen (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), whereas IgM was relatively up\u2010regulated in blood (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) after stimulation with each of the three pathogens assessed. The study is the first to report identification of IgZ in <jats:italic>C. catla<\/jats:italic>. Further, it provides insights into the differential expression profiles of IgZ and IgM isotypes against various pathogenic infection in <jats:italic>C. catla<\/jats:italic>, which may facilitate better prophylaxis again such infections.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12399","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T17:17:22Z","timestamp":1466011042000},"page":"561-567","source":"Crossref","is-referenced-by-count":11,"title":["Molecular cloning of IgZ heavy chain isotype in <i>Catla catla<\/i> and comparative expression profile of IgZ and IgM following pathogenic infection"],"prefix":"10.1111","volume":"60","author":[{"given":"Bhakti","family":"Patel","sequence":"first","affiliation":[{"name":"Laboratory of Environmental Microbiology and Ecology Department of Life Science National Institute of Technology Rourkela 769 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