{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:33:43Z","timestamp":1791304423425,"version":"4.3.1"},"reference-count":58,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,9,23]],"date-time":"2016-09-23T00:00:00Z","timestamp":1474588800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fundacao Butantan","award":["2012\/51165-7"],"award-info":[{"award-number":["2012\/51165-7"]}]},{"name":"Fundacao Butantan","award":["2014\/03792-8"],"award-info":[{"award-number":["2014\/03792-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>A severe re\u2010emergingzoonosis, leptospirosis, is caused by pathogenic spirochetes of the genus <jats:italic>Leptospira<\/jats:italic>. Several studies have identified leptospiral surface proteins with the ability to bind ECM and plasma components, which could mediate adhesion and invasion through the hosts. It has been shown that Mce of pathogenic <jats:italic>Leptospira<\/jats:italic> spp. is an RGD (Arg\u2010Gly\u2010Asp)\u2010motif\u2010dependent virulence factor, responsible for infection of cells and animals. In the present article, we decided to further study the repertoire of the Mce activities in leptospiral biological properties. We report that the recombinant Mce is a broad\u2010spectrum ECM\u2010binding protein, capable of interacting with laminin, cellular and plasma fibronectin and collagen IV. Dose\u00ad\u2013r\u00adesponse interaction was observed for all the components, fulfilling ligand\u00ad\u2013receptor requirements. Mce is a PLG binding protein capable to recruit this component from NHS, generating PLA in the presence of PLG activator. Binding of Mce was also observed with the leukocyte cell receptors \u03b1L\u03b22 [(CD11a\/CD18)\u2010LFA\u20101] and \u03b1M\u03b22 [(CD11b\/CD18)\u2010Mac\u20101], suggesting the involvement of this protein in the host immune response. Indeed, virulent <jats:italic>Leptospira<\/jats:italic> L1\u2010130 was capable of binding both integrins, whereas culture\u2010attenuated M\u201020 strain only bind to \u03b1M\u03b22 [(CD11b\/CD18)\u2010Mac\u20101]. To the best of our knowledge, this is the first work to describe that Mce surface protein could mediate the attachment of <jats:italic>Leptospira interrogans<\/jats:italic> to human cell receptors \u03b1L\u03b22(CD11a\/CD18) and \u03b1M\u03b22(CD11b\/CD18).<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12406","type":"journal-article","created":{"date-parts":[[2016,7,28]],"date-time":"2016-07-28T07:22:52Z","timestamp":1469690572000},"page":"586-598","source":"Crossref","is-referenced-by-count":20,"title":["Mammalian cell entry (Mce) protein of <i>Leptospira interrogans<\/i> binds extracellular matrix components, plasminogen and \u03b22 integrin"],"prefix":"10.1111","volume":"60","author":[{"given":"Maria Raquel","family":"Cosate","sequence":"first","affiliation":[{"name":"Biotechnology Center Butantan Institute 05503\u2013900 Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Hase","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Biotechnology Center Butantan Institute 05503\u2013900 Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele Oliveira","family":"de Souza","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science University of Sao Paulo 05508\u2013270 Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio Arruda","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science University of Sao Paulo 05508\u2013270 Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Lucia T. 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