{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T13:17:09Z","timestamp":1774099029837,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T00:00:00Z","timestamp":1477612800000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"EU structural fund ITMS"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Outer surface protein C (OspC) of <jats:italic>Borrelia<\/jats:italic> stimulates remarkable immune responses during early infection and is therefore currently considered a leading diagnostic and vaccine candidate. The sensitivity and specificity of serological tests based on whole protein OspC for diagnosis of Lyme disease are still unsatisfactory. Minimal B\u2010cell epitopes are key in the development of reliable immunodiagnostic tools. Using OspC fragments displayed on phage particles (phage library) and anti\u2010OspC antibodies isolated from sera of naturally infected patients, six OspC epitopes capable of distinguishing between LD patient and healthy control sera were identified. Three of these epitopes are located at the N\u2010terminus (OspC E1 aa19\u201327, OspC E2 aa38\u201353, OspC E3 aa62\u201366) and three at the C\u2010terminal end (OspC E4 aa155\u2013163, OspC E5 aa184\u2013190 and OspC E6 aa201\u2013207). OspC E1, E4 and E6 were highly conserved among LD related <jats:italic>Borreliae<\/jats:italic>. To our knowledge, epitopes OspC E2, E3 and E5 were identified for the first time in this study. Minimal B\u2010cell epitopes may provide fundamental data for the development of multi\u2010epitope\u2010based diagnostic tools for Lyme disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12438","type":"journal-article","created":{"date-parts":[[2016,9,13]],"date-time":"2016-09-13T10:43:27Z","timestamp":1473763407000},"page":"669-677","source":"Crossref","is-referenced-by-count":13,"title":["Identification of B\u2010cell epitopes of <i>Borrelia burgdorferi<\/i> outer surface protein C by screening a phage\u2010displayed gene fragment library"],"prefix":"10.1111","volume":"60","author":[{"given":"Lucia","family":"Pulzova","sequence":"first","affiliation":[{"name":"Laboratory of Biomedical Microbiology and Immunology Department of Microbiology and Immunology University of Veterinary Medicine and Pharmacy Komenskeho 73, 04181 Kosice 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