{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:07:45Z","timestamp":1787242065246,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,12,6]],"date-time":"2016-12-06T00:00:00Z","timestamp":1480982400000},"content-version":"vor","delay-in-days":35,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tunisian Ministry of Higher Education and Scientific Research"},{"name":"Clinical Hematology Department at Farhat Hached Hospital"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Primary infection with human herpesvirus\u20106 (HHV\u20106), is followed by its lifelong persistence in the host. Most T\u2010cell responses to HHV\u20106 have been characterized using peripheral blood from healthy adults; however, the role of HHV\u20106 infection in immune modulation has not been elucidated for some diseases. Therefore, in this study the immune response to HHV\u20106 infection in patients with B\u2010acute lymphoblastic leukemia (B\u2010ALL) was analyzed. HHV\u20106 load was quantified in blood samples taken at the time of diagnosis of leukemia and on remission. The same concentrations of anti\u2010 and pro\u2010inflammatory cytokines (IL\u20104, IL\u20101, IL\u20106, IL\u20108, IL\u201012p70, IL\u201017a, TNF\u2010\u03b1 and IFN\u2010\u03b3) were detected in plasma samples from 20 patients with and 20 without detectable HHV\u20106 virus loads in blood. Characterization of T\u2010cell responses to HHV\u20106 showed low specific T\u2010cells frequencies of 2.08% and 1.46% in patients with and without detectable viral loads, respectively. IFN\u2010\u03b3\u2010producing T cells were detected in 0.03%\u20130.23% and in 0%\u20130.2% of CD4+T cells, respectively. Strong production of IL\u20106 was detected in medium supernatants of challenged T\u2010cells whatever the HHV\u20106 status of the patients (973.51\u2009\u00b1\u2009210.06 versus 825.70\u2009\u00b1\u2009210.81\u2009pg\/mL). However, concentrations of TNF\u2010\u03b1 and IFN\u2010\u03b3 were low. Thus, no association between plasma concentrations of cytokines and detection of HHV\u20106 in blood was identified, suggesting that HHV\u20106 is not strongly associated with development of B\u2010ALL. The low viral loads detected may correspond with latently infected cells. Alternatively, HHV\u20106B specific immune responses may be below the detection threshold of the assays used.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1348-0421.12452","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T13:36:33Z","timestamp":1479303393000},"page":"770-777","source":"Crossref","is-referenced-by-count":2,"title":["Cytokine and cellular responses to human herpesvirus\u20106B in patients with B\u2010acute lymphoblastic leukemia"],"prefix":"10.1111","volume":"60","author":[{"given":"Faten","family":"Nefzi","sequence":"first","affiliation":[{"name":"Laboratory of Transmissible Diseases and Biological Active Substances, LR99ES27 Faculty of Pharmacy, University of Monastir Street Avicenne 5000 Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Lambert","sequence":"additional","affiliation":[{"name":"Immunology Laboratory, Georges Friedel Laboratory (CNRS UMR5307); University 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