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In this study, the efficacies of three different adjuvants, Freund's adjuvant (FA), aluminum hydroxide (alum) and CpG oligodeoxynucleotide (ODN), were examined from the viewpoint of inducing PcrV\u2010specific immunity against virulent <jats:italic>P. aeruginosa<\/jats:italic>. Mice that had been immunized intraperitoneally with recombinant PcrV formulated with one of the above adjuvants were challenged intratracheally with a lethal dose of <jats:italic>P. aeruginosa<\/jats:italic>. The PcrV\u2013FA immunized group attained a survival rate of 91%, whereas the survival rates of the PcrV\u2013alum and PcrV\u2013CpG groups were 73% and 64%, respectively. In terms of hypothermia recovery after bacterial instillation, PcrV\u2013alum was the most protective, followed by PcrV\u2013FA and PcrV\u2013CpG. The lung edema index was lower in the PcrV\u2013CpG vaccination group than in the other groups. PcrV\u2013alum immunization was associated with the greatest decrease in myeloperoxidase in infected lungs, and also decreased the number of lung bacteria to a similar number as in the PcrV\u2013FA group. There was less neutrophil recruitment in the lungs of mice vaccinated with PcrV\u2013alum or PcrV\u2013CpG than in those of mice vaccinated with PcrV\u2013FA or PcrV alone. Overall, in terms of mouse survival the PcrV\u2013CpG vaccine, which could be a relatively safe next\u2010generation vaccine, showed a comparable effect to the PcrV\u2013alum vaccine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12467","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T11:55:22Z","timestamp":1490788522000},"page":"64-74","source":"Crossref","is-referenced-by-count":24,"title":["Efficacy comparison of adjuvants in PcrV vaccine against <i>Pseudomonas aeruginosa<\/i> pneumonia"],"prefix":"10.1111","volume":"61","author":[{"given":"Saeko","family":"Hamaoka","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Kyoto Prefectural University of Medicine Kyoto 602\u20108566 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshifumi","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Kyoto Prefectural University of Medicine Kyoto 602\u20108566 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