{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T07:40:26Z","timestamp":1696232426964},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T00:00:00Z","timestamp":1490745600000},"content-version":"vor","delay-in-days":56,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scientific and Technical Research Council of Turkey","award":["114C095"],"award-info":[{"award-number":["114C095"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label \/><jats:p><jats:italic>Helicobacter pylori,<\/jats:italic> an important human pathogen, is capable of causing persistent infection with minimal immune response. The first line of defense during <jats:italic>H. pylori<\/jats:italic> infection is through gastric epithelial cells that present TLR, A family of bacterial proteins that share homology with the Toll\/IL\u20101 receptor (TIR) domain were identified. Bacterial TIR proteins (BTP) mimic human TIR domain proteins and act on myeloid differentiation primary response gene 88 (MyD88) signaling pathways to suppress TLR signaling. <jats:italic>H. pylori<\/jats:italic> may also produce a similar protein. A putative <jats:italic>H. pylori<\/jats:italic> BTP was found based on sequence homology. The corresponding gene <jats:italic>hp1437<\/jats:italic> was inserted into an expression vector in fusion with an N\u2010terminal cleavable 6his\u2010tag. The recombinant protein, 6his\u2010HP1437, was purified using nickel affinity chromatography with a yield of 8\u2009mg\/L culture. Oligomerization of HP1437 was investigated by size\u2010exclusion chromatography. It was found that HP1437 forms dimers in solution similar to other BTPs. Furthermore, glutathione S\u2010transferase pull down assays identified an interaction between HP1437 and human TIR domain adaptor MyD88. These findings suggest that HP1437 has the characteristic features of BTPs and may play a direct role in reducing immune response against <jats:italic>H. pylori<\/jats:italic> by binding to MyD88 and pave the way for an in\u2010depth characterization of this putative novel <jats:italic>H. pylori<\/jats:italic> virulence factor.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12469","type":"journal-article","created":{"date-parts":[[2017,3,6]],"date-time":"2017-03-06T07:52:41Z","timestamp":1488786761000},"page":"85-91","source":"Crossref","is-referenced-by-count":2,"title":["A putative Toll\/interleukin\u20101 receptor domain protein from <i>Helicobacter pylori<\/i> is dimeric in solution and interacts with human Toll\u2010like receptor adaptor myeloid differentiation primary response 88"],"prefix":"10.1111","volume":"61","author":[{"given":"Burcu","family":"Kaplan\u2010T\u00fcrk\u00f6z","sequence":"first","affiliation":[{"name":"Department of Food Engineering Faculty of Engineering 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