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Anti\u2010<jats:italic>Brucella<\/jats:italic> positive sera showed higher absorbance to <jats:italic>B. abortus<\/jats:italic> than <jats:italic>B. canis<\/jats:italic> in almost all samples. Anti\u2010<jats:italic>B. abortus<\/jats:italic> antibodies were detected in serum samples from 24% (<jats:italic>n<\/jats:italic>\u2009=\u200955) of Western Pacific harbor seals (<jats:italic>Phoca vitulina stejnegeri<\/jats:italic>) in Cape Erimo and from 66% (<jats:italic>n<\/jats:italic>\u2009=\u200941) of spotted seals (<jats:italic>P. largha<\/jats:italic>), 15% (<jats:italic>n<\/jats:italic>\u2009=\u200920) of ribbon seals (<jats:italic>Histriophoca fasciata<\/jats:italic>) and 18% (<jats:italic>n<\/jats:italic>\u2009=\u200917) of Western Steller's sea lions (<jats:italic>Eumetopias jubatus jubatus<\/jats:italic>) in the Shiretoko Peninsula. Anti\u2010<jats:italic>Brucella<\/jats:italic> antibodies were detected at higher absorbance in 1\u2010 to 4\u2010year\u2010old harbor seals than in the pups and mature animals, suggesting either that <jats:italic>Brucella<\/jats:italic> infection mainly occurs after weaning or that it is maternally transmitted to pups with premature or suppressed immunity. Anti\u2010<jats:italic>Brucella<\/jats:italic> antibodies were detected in both immature and mature spotted seals and ribbon seals, with higher absorbance in the former. The antibodies were detected only in mature Western Steller's sea lions. Western blot analysis of the serum samples showed some differences in band appearances, namely discrete versus smeary, and in the number of bands, indicating that multiple different <jats:italic>Brucella<\/jats:italic> may be prevalent in pinnipeds in Hokkaido. Alternatively, the <jats:italic>Brucella<\/jats:italic> of pinnipeds may have some intra\u2010species diversity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12474","type":"journal-article","created":{"date-parts":[[2017,3,6]],"date-time":"2017-03-06T12:27:09Z","timestamp":1488803229000},"page":"114-122","source":"Crossref","is-referenced-by-count":8,"title":["Serologic evidence of <i>Brucella<\/i> infection in pinnipeds along the coast of Hokkaido, the northernmost main island of Japan"],"prefix":"10.1111","volume":"61","author":[{"given":"Erika","family":"Abe","sequence":"first","affiliation":[{"name":"Japan Agency for Marine\u2010Earth Science and Technology 2\u201015 Natsushima\u2010cho, Yokosuka Kanagawa 237\u20100061"},{"name":"Tokyo University of Marine Science and Technology 4\u20105\u20107 Kounan, Minato\u2010ku Tokyo 108\u20108477"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazue","family":"Ohishi","sequence":"additional","affiliation":[{"name":"Japan 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