{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:51:12Z","timestamp":1790650272117,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T00:00:00Z","timestamp":1494979200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Dr Takehiko Saito of the National Institute of Animal Health"},{"name":"Dr Luk S"},{"name":"Animal Health Institute"},{"name":"Program for Leading Graduate Schools"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>H5 highly pathogenic avian influenza viruses (HPAIV) have spread in both poultry and wild birds since late 2003. Continued circulation of HPAIV in poultry in several regions of the world has led to antigenic drift. In the present study, we analyzed the antigenic properties of H5 HPAIV isolated in Asia using four neutralizing mAbs recognizing hemagglutinin, which were established using A\/chicken\/Kumamoto\/1\u20107\/2014 (H5N8), belonging to clade 2.3.4.4 and also using polyclonal antibodies. Viruses of clades 1.1, 2.3.2.1, 2.3.4, and 2.3.4.4 had different reactivity patterns to the panel of mAbs, thereby indicating that the antigenicity of the viruses of clade 2.3.4.4 were similar but differed from the other clades. In particular, the antigenicity of the viruses of clade 2.3.4.4 differed from those of the viruses of clades 2.3.4 and 2.3.2.1, which suggests that the recent H5 HPAIV have further evolved antigenically divergent. In addition, reactivity of antiserum suggests that the antigenicity of viruses of clade 2.3.4.4 differed slightly among groups A, B, and C. Vaccines are still used in poultry in endemic countries, so the antigenicity of H5 HPAIV should be monitored continually to facilitate control of avian influenza. The panel of mAbs established in the present study will be useful for detecting antigenic drift in the H5 viruses that emerge from the current strains.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12478","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T02:24:12Z","timestamp":1490927052000},"page":"149-158","source":"Crossref","is-referenced-by-count":27,"title":["Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia"],"prefix":"10.1111","volume":"61","author":[{"given":"Ayako","family":"Ohkawara","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine Hokkaido University North 18, West 9, Kita\u2010ku Sapporo Hokkaido 060\u20100818"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masatoshi","family":"Okamatsu","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine Hokkaido University North 18, West 9, Kita\u2010ku Sapporo Hokkaido 060\u20100818"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Ozawa","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Hygiene Joint Faculty of Veterinary Medicine Kagoshima University 1\u201021\u201024 Korimoto Kagoshima 890\u20100065"},{"name":"Transboundary Animal Diseases Center Joint Faculty of Veterinary Medicine, Kagoshima University 1\u201021\u201024 Korimoto Kagoshima 890\u20100065"},{"name":"United Graduate School of Veterinary Science Yamaguchi University 1677\u20101 Yoshida Yamaguchi 753\u20108515"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc\u2010Huy","family":"Chu","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine Hokkaido University North 18, West 9, Kita\u2010ku Sapporo Hokkaido 060\u20100818"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lam Thanh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine Hokkaido University North 18, West 9, Kita\u2010ku Sapporo Hokkaido 060\u20100818"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Hiono","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine Hokkaido University North 18, West 9, Kita\u2010ku Sapporo Hokkaido 060\u20100818"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Matsuno","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine Hokkaido University North 18, West 9, Kita\u2010ku Sapporo Hokkaido 060\u20100818"},{"name":"Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI\u2010CoRE) Hokkaido University North 20, West 10, Kita\u2010ku, Sapporo Hokkaido 001\u20100020 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kida","sequence":"additional","affiliation":[{"name":"Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI\u2010CoRE) Hokkaido University North 20, West 10, Kita\u2010ku, Sapporo Hokkaido 001\u20100020 Japan"},{"name":"Research Center for Zoonosis Control Hokkaido University North 20, West 10, Kita\u2010ku Sapporo Hokkaido 001\u20100020 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Sakoda","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine Hokkaido University North 18, West 9, Kita\u2010ku Sapporo Hokkaido 060\u20100818"},{"name":"Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI\u2010CoRE) Hokkaido University North 20, West 10, Kita\u2010ku, Sapporo Hokkaido 001\u20100020 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9820"},{"key":"e_1_2_8_3_1","unstructured":"Web portal on Avian Influenza: OIE \u2212 World Organisation for Animal Health UPDATE ON HIGHLY PATHOGENIC AVIAN INFLUENZA IN ANIMALS (TYPE H5 and H7). (6 June 2016) Available from:http:\/\/www.oie.int\/animal\u2010health\u2010in\u2010the\u2010world\/"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/irv.12324","article-title":"World Health Organization\/World Organization for Animal Health\/Food and Agriculture Organization (WHO\/OIE\/FAO) H5 Evolution Working Group. (2015) Nomenclature updates resulting from the evolution of avian influenza A(H5) virus clades 2.1.3.2a, 2.2.1, and 2.3.4 during 2013\u20102014","volume":"9","author":"Smith G.J.","journal-title":"Influenza Other Respir Viruses"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2015.20.20.21132"},{"key":"e_1_2_8_6_1","first-page":"1190","volume-title":"Fields Virology","author":"Wright P.F.","year":"2013"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-015-1214-9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-015-2428-9"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90135-0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/289373a0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-10-2497"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124513"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.5.865"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/594374"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00465-08"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1566"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1857"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00754"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.12.006"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2207.160048"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12369"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.11.001"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(82)90375-0"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-008-0226-3"},{"key":"e_1_2_8_26_1","first-page":"415","article-title":"Antigenic relationship between influenza A viruses of human and animal origin","volume":"38","author":"Tumov\u00e1 B.","year":"1968","journal-title":"Bull World Health Organ"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.02.001"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050170002"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1236787"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/289366a0"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.08.033"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005996"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-65"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14234"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03014-14"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004566"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.11.022"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-015-4642-2"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.04.016"},{"key":"e_1_2_8_40_1","first-page":"79","article-title":"Current status and future needs in diagnostics and vaccines for high pathogenicity avian influenza","volume":"135","author":"Swayne D.E.","year":"2013","journal-title":"Dev Biol (Basel)"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12478","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12478","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T12:51:02Z","timestamp":1696510262000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1111\/1348-0421.12478"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12478","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5]]}}}