{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:40:51Z","timestamp":1790692851571,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T00:00:00Z","timestamp":1497312000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>JC polyomavirus (JCPyV) is the causative agent of the demyelinating disease of the central nervous system known as progressive multifocal leukoencephalopathy (PML), which occurs in immunocompromised patients. Moreover, patients treated with natalizumab for multiple sclerosis or Crohn disease can develop PML, which is then termed natalizumab\u2010related PML. Because few drugs are currently available for treating PML, many antiviral agents are being investigated. It has been demonstrated that the topoisomerase I inhibitors topotecan and \u03b2\u2010lapachone have inhibitory effects on JCPyV replication in IMR\u201032 cells. However, both of these drugs have marginal inhibitory effects on virus propagation in JC1 cells according to RT\u2010PCR analysis. In the present study, the inhibitory effect of another topoisomerase I inhibitor, 7\u2010ethy\u201010\u2010[4\u2010(1\u2010piperidino)\u20101\u2010piperidino] carbonyloxy camptothecin (CPT11), was assessed by investigating viral replication, propagation, and viral protein 1 (VP1) production in cultured cells. JCPyV replication was assayed using real\u2010time PCR combined with <jats:italic>Dpn<\/jats:italic> I treatment in IMR\u201032 cells transfected with JCPyV DNA. It was found that JCPyV replicates less in IMR\u201032 cells treated with CPT11 than in untreated cells. Moreover, CPT11 treatment of JCI cells persistently infected with JCPyV led to a dose\u2010dependent reduction in JCPyV DNA and VP1 production. Additionally, the inhibitory effect of CPT11 was found to be stronger than those of topotecan and \u03b2\u2010lapachone. These findings suggest that CPT11 may be a potential anti\u2010JCPyV agent that could be used to treat PML.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12486","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T15:29:42Z","timestamp":1493738982000},"page":"232-238","source":"Crossref","is-referenced-by-count":4,"title":["CPT11 prevents virus replication in JCI cells persistently infected with JC polyomavirus"],"prefix":"10.1111","volume":"61","author":[{"given":"Souichi","family":"Nukuzuma","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Kobe Institute of Health 4\u20106\u20105, Minatojima\u2010Nakamachi, Chuo\u2010ku, Kobe 650\u20100046 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiyoko","family":"Nukuzuma","sequence":"additional","affiliation":[{"name":"Thermocell Shinagawa\u2010ku, Tokyo 141\u20100022 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Kameoka","sequence":"additional","affiliation":[{"name":"Department of International Health Kobe University Graduate School of Health Sciences Suma\u2010ku, Kobe 615\u20100124 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeki","family":"Sugiura","sequence":"additional","affiliation":[{"name":"Medical Genetics Research Center Nara Medical University Kashihara, Nara 634\u20108521 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Nakamichi","sequence":"additional","affiliation":[{"name":"Department of Virology 1 National Institute of Infectious Diseases, Toyama, Shinjuku Tokyo 162\u20108640 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takafumi","family":"Tasaki","sequence":"additional","affiliation":[{"name":"Divison of Protein Regulation Research Medical Research Institute, Kanazawa Medical University 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