{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T06:42:53Z","timestamp":1771051373367,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T00:00:00Z","timestamp":1497312000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Japanese Ministry of Agriculture, Forestry and Fisheries"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Erythromycin, tylosin and tilmicosin are approved for use in cattle in Japan, the latter two being used to treat <jats:italic>Mycoplasma bovis<\/jats:italic> infection. In this study, 58\u2009<jats:italic>M. bovis<\/jats:italic> isolates obtained from Japanese dairy calves all exhibited reduced susceptibility to these macrolides, this widespread reduced susceptibility being attributable to a few dominant lineages. All 58 isolates contained the G748A variant in both the <jats:italic>rrl3<\/jats:italic> and <jats:italic>rrl4<\/jats:italic> alleles of 23S rRNA, whereas a reference strain (PG45) did not. G748 localizes in the central loop of domain II (from C744 to A753) of 23S rRNA, which participates in binding to mycinose, a sugar residue present in both tylosin and tilmicosin. A number of <jats:italic>in vitro\u2010<\/jats:italic>selected mutants derived from <jats:italic>M. bovis<\/jats:italic> PG45 showed reduced susceptibility to tylosin and tilmicosin and contained a nucleotide insertion within the central loop of domain II of <jats:italic>rrl3<\/jats:italic> (U747\u2013G748Ins_CU\/GU or A743\u2013U744Ins_UA), suggesting that mutations around G748 confer this reduced susceptibility phenotype. However, other <jats:italic>Mycoplasma<\/jats:italic> species containing G748A were susceptible to tylosin and tilmicosin. Sequence comparison with <jats:italic>Escherichia coli<\/jats:italic> revealed that <jats:italic>M. bovis<\/jats:italic> PG45 and isolates harbored five nucleotide alterations (U744C, G745A, U746C, A752C and A753G) in the central loop of domain II of 23S rRNA, whereas other <jats:italic>Mycoplasma<\/jats:italic> species lacked at least two of these five nucleotide alterations. It was therefore concluded that G748 mutations in combination with species\u2010specific nucleotide alterations in the central loop of domain II of 23S rRNA are likely sufficient to reduce susceptibility of <jats:italic>M. bovis<\/jats:italic> to tylosin and tilmicosin.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12490","type":"journal-article","created":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T10:24:44Z","timestamp":1494843884000},"page":"215-224","source":"Crossref","is-referenced-by-count":11,"title":["<i>Mycoplasma bovis<\/i> isolates from dairy calves in Japan have less susceptibility than a reference strain to all approved macrolides associated with a point mutation (G748A) combined with multiple species\u2010specific nucleotide alterations in 23S rRNA"],"prefix":"10.1111","volume":"61","author":[{"given":"Toyotaka","family":"Sato","sequence":"first","affiliation":[{"name":"Laboratory of Food Microbiology and Food Safety Department of Health and Environmental Sciences School of Veterinary Medicine Rakuno Gakuen University 582 Bunkyoudai\u2010Midorimachi, Ebetsu, 069\u20108501 Japan"},{"name":"Department of Microbiology Sapporo Medical University School of Medicine S1 W17, Chuo\u2010ku, Sapporo, 060\u20108556 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetoshi","family":"Higuchi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Health Department of Health and Environmental Sciences School of Veterinary Medicine Rakuno Gakuen University Ebetsu 069\u20108501 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010ichi","family":"Yokota","sequence":"additional","affiliation":[{"name":"Department of Microbiology Sapporo Medical University School of Medicine S1 W17, Chuo\u2010ku, Sapporo, 060\u20108556 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Tamura","sequence":"additional","affiliation":[{"name":"Laboratory of Food Microbiology and Food Safety Department of Health and Environmental Sciences School of Veterinary Medicine Rakuno Gakuen University 582 Bunkyoudai\u2010Midorimachi, Ebetsu, 069\u20108501 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1438-4221(00)80099-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870601800104"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.42.1253"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-4-1093"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.10.3142-3150.2002"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00726-10"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.10-0165"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.102303"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087672"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12144"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-014-0256-x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00595"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700505"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.01.028"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.11.033"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.15-0304"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.suppl_2.1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01841.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05975.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.10.2958-2960.2001"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.67.795"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.05.012"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.12-0508"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.d5331"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2000100"},{"key":"e_1_2_8_27_1","unstructured":"Clinical and Laboratory Standards Institute. (2015) Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard\u2010Fourth Edition and Supplement. VET01A4E and VET01S3E. W. Pennsylvania USA:CLSI."},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4556-4565.2004"},{"key":"e_1_2_8_29_1","unstructured":"CentroidFold. [Cited\u2026.] Available from URL:http:\/\/www.ncrna.org\/centroidfold"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp228"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3677-3683.2004"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.232580599"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02046.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.3.672-679.2002"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01202.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.12.2823"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4624-4630.2004"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-42-90"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05516-11"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.11.4470-4472.2004"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00420-07"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1348-0421.12490","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1348-0421.12490","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T09:34:34Z","timestamp":1694338474000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1348-0421.12490"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/1348-0421.12490"],"URL":"https:\/\/doi.org\/10.1111\/1348-0421.12490","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6]]}}}