{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T22:54:07Z","timestamp":1776725647100,"version":"3.51.2"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,8,16]],"date-time":"2017-08-16T00:00:00Z","timestamp":1502841600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81360290"],"award-info":[{"award-number":["81360290"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Cholangiocarcinoma (CCA) associated with liver fluke infection involves inflammatory and immune processes; however, whether these involve the proinflammatory cytokine IL\u201017A and proliferative cytokine IL\u201022 remains unclear. Here, numbers of IL\u201022\u2010 and IL\u201017A\u2010producing Th cells and cytokine concentrations in 30 patients with CCA and long\u2010term liver fluke infection, 40 patients with liver\u2010fluke infection but not CCA, and 16 healthy controls were compared. Analyses were performed using immunohistochemistry, flow cytometry, ELISA and RT\u2010PCR. Immunohistochemical staining showed weaker expression of IL\u201022 and IL\u201017A in patients with CCA with than in those without liver fluke infection (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). Flow cytometry revealed significantly greater median proportions of IL\u201022\u2010producing T helper cells in patients with CCA (2.2%) than in those without it (0.69%) or controls (0.4%, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Similar results were obtained for IL\u201017A\u2010producing T helper cells. ELISA revealed plasma concentrations of IL\u201022 were 1.3\u2010fold higher in patients with CCA than in those without it and 4.6\u2010fold higher than in controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Plasma concentrations of IL\u201017A were 2.5\u2010fold higher in patients with CCA than in those without it, and 21\u2010fold higher than in controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Amounts of IL\u201022 and IL\u201017A mRNAs in blood were significantly higher in patients with CCA than in the other two groups. Proportions of CD4<jats:sup>+<\/jats:sup>CD45RO<jats:sup>+<\/jats:sup> T cells producing IL\u201022 correlated with proportions producing IL\u201017A (r\u2009=\u20090.759; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), and plasma concentrations of IL\u201022 correlated with those of IL\u201017A (r\u2009=\u20090.726; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). These results suggest that both IL\u201017A and IL\u201022 affect development of CCA related to liver fluke infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12500","type":"journal-article","created":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T13:05:51Z","timestamp":1500383151000},"page":"345-354","source":"Crossref","is-referenced-by-count":11,"title":["Large numbers of interleukins\u201022\u2010 and \u201017A\u2010producing T helper cells in cholangiocarcinoma related to liver fluke infection"],"prefix":"10.1111","volume":"61","author":[{"given":"Si\u2010Biao","family":"Su","sequence":"first","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Guangxi Medical University Nanning 530007 Guangxi Zhuang Autonomous Region China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine Second Affiliated Hospital of Guangxi Medical University Nanning 530007 Guangxi Zhuang Autonomous Region China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei\u2010Fei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Guangxi Medical University Nanning 530007 Guangxi Zhuang Autonomous Region China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Cen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Guangxi Medical University Nanning 530007 Guangxi Zhuang Autonomous Region China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010Xing","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Guangxi Medical University Nanning 530007 Guangxi Zhuang Autonomous Region 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