{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T19:05:52Z","timestamp":1761419152917},"reference-count":65,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T00:00:00Z","timestamp":1505865600000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001744","name":"International Science and Technology Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001744","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p><jats:italic>Streptococcus pneumoniae<\/jats:italic>, a common human pathogen, colonizes the nasopharynx and causes diseases including acute otitis media (AOM). Herein, pneumococcal serotype distributions in children before and after PCV7 vaccination and in patients with pneumococcal disease in Siberian Russia (Krasnoyarsk) are reported. Analyses included antimicrobial susceptibility testing, sequence typing (ST), pulsed field gel electrophoresis, virulence\u2010related surface protein gene (VSG) typing with novel primers and structural analysis by scanning electron microscopy. In healthy children (HC) prior to administration of PCV7, drug\u2010susceptible serotype23F\/ST1500 was a major pneumococcal genotype. In the PCV7 trial, multidrug\u2010resistant serotype19A\/ST320 emerged in vaccinees after PCV7, exhibiting a PCV7\u2010induced serotype replacement. Multidrug\u2010resistant serotype19A\/ST320 was evident in patients with AOM. Community\u2010acquired pneumonia (CAP) isolates showed genetic similarities to the AOM (ST320) genotype, constituting a common non\u2010invasive AOM\u2013CAP group. In contrast, meningitis isolates were more divergent. Overall, 25 ST types were identified; five (20%) of which were Krasnoyarsk\u2010native. Regarding VSGs, PI\u20101 (<jats:italic>rlrA<\/jats:italic>\/<jats:italic>rrgB<\/jats:italic>), PI\u20102 (<jats:italic>pitA<\/jats:italic>\/<jats:italic>B<\/jats:italic>), <jats:italic>psrP<\/jats:italic> and <jats:italic>cbpA<\/jats:italic> were present at 54.3%, 38.6%, 48.6%, and 95.7%, respectively, with two major VSG content types, PI\u20101<jats:sup>\u2212<\/jats:sup>\/PI\u20102<jats:sup>\u2212<\/jats:sup>\/<jats:italic>psrP<\/jats:italic><jats:sup>+<\/jats:sup>\/<jats:italic>cbpA<\/jats:italic><jats:sup>+<\/jats:sup> and PI\u20101<jats:sup>+<\/jats:sup>\/PI\u20102<jats:sup>+<\/jats:sup>\/<jats:italic>psrP<\/jats:italic><jats:sup>\u2010<\/jats:sup>\/<jats:italic>cbpA<\/jats:italic><jats:sup>+<\/jats:sup>, being found for HC and non\u2010invasive diseases, respectively. A major clone of serotype19A\/ST320 (PI\u20101<jats:sup>+<\/jats:sup>\/PI\u20102<jats:sup>+<\/jats:sup>) produced the longest pneumococcal wire (pilus) structures in colonies. ST1016 (PI\u20101<jats:sup>\u2212<\/jats:sup>\/PI\u20102<jats:sup>\u2212<\/jats:sup>) in HC had HEp\u20102 cell\u2010adherent pili. These results suggest that serotype19A\/ST320 and related genotypes, with the VSG content type PI\u20101<jats:sup>+<\/jats:sup>\/PI\u20102<jats:sup>+<\/jats:sup>\/<jats:italic>psrP<\/jats:italic><jats:sup>\u2212<\/jats:sup>\/<jats:italic>cbpA<\/jats:italic><jats:sup>+<\/jats:sup>, emerged in vaccinees after PCV7 in Siberia, accompanying diseases in non\u2010vaccinated children, and that some genotypes (serotypes19A\/ST320 and 18\/ST1016) produced novel pneumococcal structures, predicting their roles in colony formation and adherence.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12503","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T04:32:46Z","timestamp":1500870766000},"page":"359-370","source":"Crossref","is-referenced-by-count":5,"title":["Molecular characterization of <i>Streptococcus pneumoniae<\/i>, particularly serotype19A\/ST320, which emerged in Krasnoyarsk, Russia"],"prefix":"10.1111","volume":"61","author":[{"given":"Irina N.","family":"Protasova","sequence":"first","affiliation":[{"name":"Russia\u2013Japan Center of Microbiology, Metagenomics and Infectious Diseases Krasnoyarsk Russia"},{"name":"Department of Microbiology Krasnoyarsk State Medical University Krasnoyarsk Russia"},{"name":"Department of Epidemiology, Genomics, and Evolution International Medical Education and Research, Center Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsai\u2010Wen","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Genomics, and Evolution International Medical Education and Research, Center Niigata Japan"},{"name":"Department of Clinical Laboratory Sciences and Medical Biotechnology National Taiwan University College of Medicine Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalya V.","family":"Bakhareva","sequence":"additional","affiliation":[{"name":"Krasnoyarsk State Ministry of Healthcare Krasnoyarsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Chun","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Genomics, and Evolution International Medical Education and Research, Center Niigata Japan"},{"name":"Department of Microbiology and Immunology Kaohsiung Medical University Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Higuchi","sequence":"additional","affiliation":[{"name":"Division of Bacteriology Department of Infectious Disease Control and International Medicine Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhisa","family":"Iwao","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Genomics, and Evolution International Medical Education and Research, Center Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana A.","family":"Yelistratova","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Krasnoyarsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalya A.","family":"Ilyenkova","sequence":"additional","affiliation":[{"name":"Department of Children Diseases Krasnoyarsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yelena S.","family":"Sokolovskaya","sequence":"additional","affiliation":[{"name":"Department of Children Diseases Krasnoyarsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina P.","family":"Martynova","sequence":"additional","affiliation":[{"name":"Department of Children Infectious Diseases, Krasnoyarsk State Medical University Krasnoyarsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan V.","family":"Reva","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Genomics, and Evolution International Medical Education and Research, Center Niigata Japan"},{"name":"Department of Clinical and Fundamental Medicine Far Eastern Federal University School of Biomedicine Vladivostok Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina V.","family":"Reva","sequence":"additional","affiliation":[{"name":"Department of Clinical and Fundamental Medicine Far Eastern Federal University School of Biomedicine Vladivostok Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey V.","family":"Sidorenko","sequence":"additional","affiliation":[{"name":"Research Institute of Children Infections of Federal State Medical and Biological Agency of Russia Saint Petersburg"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee\u2010Jene","family":"Teng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences and Medical Biotechnology National Taiwan University College of Medicine Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga V.","family":"Peryanova","sequence":"additional","affiliation":[{"name":"Russia\u2013Japan Center of Microbiology, Metagenomics and Infectious Diseases Krasnoyarsk Russia"},{"name":"Department of Microbiology Krasnoyarsk State Medical University Krasnoyarsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alla B.","family":"Salmina","sequence":"additional","affiliation":[{"name":"Research Institute of Molecular Medicine and Pathobiochemistry Krasnoyarsk State Medical University Krasnoyarsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Russia\u2013Japan Center of Microbiology, Metagenomics and Infectious Diseases Krasnoyarsk Russia"},{"name":"Department of Epidemiology, Genomics, and Evolution International Medical Education and Research, Center Niigata 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