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The number incidence of whooping cough cases is increasing in many countries despite high vaccine coverage. Causes for the re\u2010emergence of the disease include the limited duration of protection conferred by the acellular pertussis vaccines (aP)s and pathogenic adaptations that involve antigenic divergence from vaccine strains. Therefore, current vaccines therefore need to be improved. In the present study, we focused on five autotransporters: namely SphB1, BatB, SphB2, Phg, and Vag8, which were previously found to be expressed by <jats:italic>B. bronchiseptica<\/jats:italic> during the course of infection in rats and examined their protective efficiencies as vaccine antigens. The passenger domains of these proteins were produced in recombinant forms and used as antigens. An intranasal murine challenge assay showed that immunization with a mixture of SphB1 and Vag8 (SV) significantly reduced bacterial load in the lower respiratory tract and a combination of aP and SV acts synergistically in effects of conferring protection against <jats:italic>B. pertussis<\/jats:italic> infection, implying that these antigens have potential as components to for improvinge th the currently available acellular pertussis vaccine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12504","type":"journal-article","created":{"date-parts":[[2017,7,28]],"date-time":"2017-07-28T10:36:41Z","timestamp":1501238201000},"page":"371-379","source":"Crossref","is-referenced-by-count":17,"title":["Protective effects of <i>in vivo<\/i>\u2010expressed autotransporters against <i>Bordetella pertussis<\/i> infection"],"prefix":"10.1111","volume":"61","author":[{"given":"Koichiro","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Molecular Bacteriology Research 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