{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T01:23:19Z","timestamp":1767921799905,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T00:00:00Z","timestamp":1507766400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Research Committee of \u00d6rebro County Council"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>To improve management of <jats:italic>Staphylococcus aureus<\/jats:italic> bacteremia (SAB), better understanding of host\u2013pathogen interactions is needed. <jats:italic>In vitro<\/jats:italic> studies have shown that <jats:italic>S. aureus<\/jats:italic> bacteria induce dose\u2010dependent immunosuppression that is evidenced by reduced expression of major histocompatibility complex (MHC) class II on antigen presenting cells. Thus, the aim of this study was to determine whether expression of the MHC class II\u2010related genes <jats:italic>HLA\u2010DRA<\/jats:italic> and <jats:italic>CD74<\/jats:italic> is more greatly reduced in complicated SAB, with its probable higher loads of <jats:italic>S. aureus<\/jats:italic>, than in uncomplicated SAB. Adult patients with SAB were prospectively included and blood samples taken on the day of confirmation of SAB (Day 1) and on Days 2, 3, 5 and 7. <jats:italic>HLA\u2010DRA<\/jats:italic> and <jats:italic>CD74<\/jats:italic> mRNA expression was determined by quantitative reverse transcription PCR. Sepsis was defined according to the Sepsis\u20103 classification and SAB was categorized as complicated in patients with deep\u2010seated infection and\/or hematogenous seeding. Twenty patients with SAB were enrolled and samples obtained on all assessment days. <jats:italic>HLA\u2010DRA<\/jats:italic> and <jats:italic>CD74<\/jats:italic> expression did not differ significantly between patients with SAB and sepsis (<jats:italic>n<\/jats:italic>\u2009=\u200913) and those without sepsis (<jats:italic>n<\/jats:italic>\u2009=\u20097) on any assessment day. However, patients with complicated SAB (<jats:italic>n<\/jats:italic>\u2009=\u200914) had significantly weaker <jats:italic>HLA\u2010DRA<\/jats:italic> expression on all five assessment days than patients with uncomplicated SAB (<jats:italic>n<\/jats:italic>\u2009=\u20096). Additionally, they tended to have weaker <jats:italic>CD74<\/jats:italic> expressions. Neutrophil, monocyte and leukocyte counts did not differ significantly between complicated and uncomplicated SAB. In conclusion, patients with complicated SAB show weaker <jats:italic>HLA\u2010DRA<\/jats:italic> expression than those with uncomplicated SAB during the first week of bacteremia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1348-0421.12533","type":"journal-article","created":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T11:23:02Z","timestamp":1504264982000},"page":"442-451","source":"Crossref","is-referenced-by-count":6,"title":["Expression of <i>HLA\u2010DRA<\/i> and <i>CD74<\/i> mRNA in whole blood during the course of complicated and uncomplicated <i>Staphylococcus aureus<\/i> bacteremia"],"prefix":"10.1111","volume":"61","author":[{"given":"Gunl\u00f6g","family":"Rasmussen","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases \u00d6rebro University Hospital \u00d6rebro University \u00d6rebro"},{"name":"Faculty of Medicine and Health School of Medical Sciences \u00d6rebro University 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